| 烟草叶片总RNA提取方法的比较 |
| |
| 引用本文： | 朱晓宇,姚珊珊,赵二卫,赵晨楠,崔红. 烟草叶片总RNA提取方法的比较[J]. 安徽农业科学, 2010, 38(15): 7821-7823. DOI: 10.3969/j.issn.0517-6611.2010.15.044 |
| |
| 作者姓名： | 朱晓宇 姚珊珊 赵二卫 赵晨楠 崔红 |
| |
| 作者单位： | 河南农业大学烟草学院,烟草行业栽培重点实验室,河南郑州,450002;河南农业大学烟草学院,烟草行业栽培重点实验室,河南郑州,450002;河南农业大学烟草学院,烟草行业栽培重点实验室,河南郑州,450002;河南农业大学烟草学院,烟草行业栽培重点实验室,河南郑州,450002;河南农业大学烟草学院,烟草行业栽培重点实验室,河南郑州,450002 |
| |
| 摘 要： | [目的]研究提取烟草叶片总RNA的最佳方法。[方法]利用CTAB法、酚-SDS法和Trizol法等从烟草叶片中提取总RNA。[结果]通过紫外分光光度法和凝胶电泳检测,结果表明,CTAB法、Trizol法、RNAiso法均能得到质量较高的RNA。RNAiso法提取RNA操作步骤简单,所提取的RNA纯度好、质量高且无污染,可以进行下一步的分子生物学研究。[结论]RNAiso法最适用于烟草叶片总RNA的提取。
|
| 关 键 词： | 烟草叶片 RNA提取 凝胶电泳 |
Comparison on Total RNA Extraction Methods from Tobacco Leaves |
| |
| ZHU Xiao-yu et al. Comparison on Total RNA Extraction Methods from Tobacco Leaves[J]. Journal of Anhui Agricultural Sciences, 2010, 38(15): 7821-7823. DOI: 10.3969/j.issn.0517-6611.2010.15.044 |
| |
| Authors: | ZHU Xiao-yu et al |
| |
| Affiliation: | ZHU Xiao-yu et al(College of Tobacco,Henan Agricultural University,Zhengzhou,Henan 450002) |
| |
| Abstract: | [Objective] The aim was to study the best way to extract total RNA from tobacco leaves.[Method] Modified CTAB method,phenol-SDS method and Trizol method,etc.were used to extract total RNA from tobacco leaves.[Result] The quality and quantity of total RNA were compared among five methods through UV spectrometer and gel electrophoresis.The results indicated that the CTAB method,Trizol method and RNAiso method could extract higher quality RNA from tobacco leaves.RNAiso method was easy to operate,the extracted ... |
| |
| Keywords: | Tobacco leaves RNA Extraction Gel electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
