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Proposals of the working group "Antibiotic resistance" for the configuration of microtitre plates to be used in routine antimicrobial susceptibility testing of bacterial pathogens from infections of large food-producing animals and mastitis cases
Authors:Luhofer Gabriele,Böttner Alexander,Hafez H Mohamed,Kaske Martin,Kehrenberg Corinna,Kietzmann Manfred,Klarmann Dieter,Klein Günter,Krabisch Peter,Kühn Tilman,Richter Angelika,Sigge Claudia,Traeder Wolfgang,Waldmann Karl-Heinz,Wallmann Jürgen,Werckenthin Christiane,Schwarz Stefan  Arbeitsgruppe "  Antibiotikaresistenz"  
Affiliation:Landesuntersuchungsamt Rheinland-Pfalz, Institut für Lebensmittel tierischer Herkunft, Koblenz.
Abstract:Two layouts for microtitre plates, which should serve for in-vitro susceptibility testing in routine diagnostics, have been set up by the working group "Antibiotic resistance" of the German Society for Veterinary Medicine. One of these layouts was designed for the testing of bacteria from cases of mastitis and the other for bacteria from infections in large food-producing animals. The choice of the antimicrobial agents and their concentrations to be included in these layouts were based on (1) the bacteria frequently associated with the respective diseases/animals, (2) the antimicrobial agents licensed for therapeutic use in these diseases/animals, (3) the currently available breakpoints, and (4) cross-resistances between the antimicrobial agerts so far known to occur in the respective bacteria.
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