Blastogenic responses and production of TCGF by mononuclear cells isolated from the bovine endometrium |
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| Authors: | E D Watson H G Zanecosky |
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| Affiliation: | Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348. |
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| Abstract: | Mononuclear cells (MNCs) were isolated from the endometrium of cattle by enzymatic digestion followed by centrifugation on a density gradient. Of the cells isolated, 32 +/- 3.2% rosetted AET-treated sheep red blood cells, 9 +/- 0.4% possessed surface membrane immunoglobulin, and 15 +/- 6.1% were esterase positive. The cells possessed the ability to proliferate in the presence of T cell mitogens and produced T cell growth factor (TCGF) after in vitro stimulation with Concanavalin A. Endometrial MNCs did not appear to suppress the blastogenesis of peripheral blood MNCs. |
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