| 一个棉花血红素加氧酶基因的克隆及特性分析 |
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| 引用本文： | 谢永芳,蔡应繁,冉静,李嘉平,王将,李红辉,许舰. 一个棉花血红素加氧酶基因的克隆及特性分析[J]. 江苏农业学报, 2009, 25(3) |
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| 作者姓名： | 谢永芳 蔡应繁 冉静 李嘉平 王将 李红辉 许舰 |
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| 作者单位： | 重庆邮电大学生物信息学院,重庆,400065 |
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| 基金项目： | 国家自然科学基金，重庆市自然基金 |
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| 摘 要： | 血红素加氧酶(HO)是催化血红素降解的微粒酶系统,根据HO基因的保守序列设计PCR引物,对构建棉花腺体形成时期的cDNA均一化文库PCR进行筛选,分析确定阳性克隆中cDNA片段的大小,克隆了棉花中1个HO基因全长cDNA,命名为HOCG(GenBank登记号:EU373020),并对其编码的氨基酸序列进行分析.结果显示,HOCG基因长度为1 462 bp,编码318个氨基酸,其编码的蛋白质预测的等电点和分子量分别为5 580和36 220.RT-PCR分析表明,该基因在湘棉18腺体形成前后表达有差异,该基因的克隆与分析有利于进一步探明棉花腺体形成的机制.
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| 关 键 词： | 棉花 血红素加氧酶 序列分析 |
Cloning and Characterization of A Full-length HO Gene in Cotton |
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| XIE Yong-fang,CAI Ying-fan,RAN Jing,LI Jia-ping,WANG Jiang,LI Hong-hui,XU Jian. Cloning and Characterization of A Full-length HO Gene in Cotton[J]. Jiangsu Journal of Agricultural Sciences, 2009, 25(3) |
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| Authors: | XIE Yong-fang CAI Ying-fan RAN Jing LI Jia-ping WANG Jiang LI Hong-hui XU Jian |
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| Affiliation: | College of Bioinformation;Chongqing University of Posts and Communications;Chongqing 400065;China |
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| Abstract: | HO is a kind of microparticle enzyme to catalysis degradation of heme.A pair of PCR primers was designed according to conserved sequences of HO family gene of different varieties.The normolization library was screened and the inserted cDNA lengths of positive clones were analysed by PCR.While the largest inserted cDNA was sequenced,a full-length cDNA of HO was obtained(GenBank accession number:EU373020)in cotton.The length of HOCG is 1 462 bp,which contains an open reading frame encoding 318 amino acid and ... |
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| Keywords: | cotton HO characterization |
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