Cloning and characterization of the glutamine synthetase gene from Chinese shrimp <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> |
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Authors: | Xiao Fang Lai Huan Gao Jie Kong Qing Yin Wang Wei Ji Wang Xian Hong Meng |
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Institution: | (1) Jiangsu Key Laboratory of Marine Biotechnology, 222005 Lianyungang, Jiangsu, China;(2) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, Shandong, China; |
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Abstract: | Glutamine synthetase (GS) is considered to be one of the oldest existing functioning genes in evolution and plays a key role
in two major biochemical pathways: in liver GS catalyzes ammonia detoxification, whereas in neural tissues it also functions
in recycling of the neurotransmitter glutamate. In this study, a cytosolic glutamine synthetase was cloned and characterized
from the Chinese Shrimp Fenneropenaeus chinensis, and named as FcGS. The results indicated that the full-length DNA of 1,756 bp had an open reading frame without intron,
which encoded a polypeptide of 366 amino acids with several conserved active site residues. The calculated molecular mass
of mature protein was 40.7 kDa and theoretical pI was 6.47. A phylogenetic analysis of GS sequences showed that FcGS clustered
with the invertebrate group as expected. qRT-PCR analysis indicated that FcGS was expressed in intestines, hepatopancreas,
muscles and gills, and its profile was up-regulated post-WSSV challenge in hepatopancreas and gills. Our results suggested
that FcGS might participate in the regulation of shrimp immune response toward WSSV invasion. These data would be helpful
to better understand the WSSV resistance mechanism of farming shrimps. |
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