菌剂配施有机肥下植物-土壤-微生物生态化学计量特征及内稳性——以内蒙矿区排土场中药复垦模式为例

生态化学计量学是研究生态系统与多重化学元素平衡的有效方法，明确不同施肥制度对植物—土壤—微生物连续体碳、氮、磷含量及其生态化学计量比的影响，可为揭示生态系统养分循环、实现矿山复垦农业系统元素平衡及可持续发展提供参考依据。以武家塔露天煤矿排土场复垦地为研究对象，设置微生物菌剂配施不同有机肥(A1B0、A2B0、A3B0、A1B1、A2B1、A3B1) 6个施肥处理，结合苦参的种植，研究其植物—土壤—微生物碳氮磷特征及内稳性。结果表明：(1)与单施有机肥相比，有机肥配施微生物菌剂对茎叶磷、土壤碳氮磷和微生物量氮影响显著(p<0.05),但对根系养分影响不显著。其中，微生物菌剂与A1有机肥配施下对土壤全磷影响最大，增加90.06%;与A2有机肥配施下，对土壤有机碳和生物量氮影响最大，分别增加104.60%和71.95%;与A3有机肥配施下，对茎叶全磷和土壤全氮影响显著，茎叶全磷减少183.96%,土壤全氮增加29.14%。(2)施肥处理下，相比于茎叶和微生物，苦参根系内稳性较弱，对于外源养分的输入比较敏感。(3)施肥处理下，植物根系养分与土壤及土壤微生物之间相关性较强，因此可通过根系敏...

Ecostoichiometric Characteristics and Internal Stability of Plant-Soil-Microbial Ecosystem Under Organic Fertilizer Application with Fungicides-An Example of Traditional Chinese Medicine Reclamation Model in A Mining Site in Inner Mongolia

Ecochemometrics is an effective method to study the balance of ecosystem and multiple chemical elements, and to clarify the impact of different fertilization systems on the carbon, nitrogen, phosphorus content and their ecostoichiometric ratio of plant-soil-microbial continuum, which can provide a feasible basis for revealing the nutrient cycle of ecosystem and realizing the element balance and sustainable development of mining reclamation agricultural system. In this study, the reclaimed land of the waste dump of Wujiata open-pit coal mine was taken as the research object, and six fertilization treatments were set up, including single application of organic fertilizer with three green manure ratios (A1B0, A2B0, A3B0) and microbial inoculum with different organic fertilizers (A1B1, A2B1, A3B1). The characteristics of carbon, nitrogen and phosphorus of plant-soil-microbial ecosystem were studied in combination with the planting of Sophora flavescens. The results showed that:(1) Compared with the single application of organic fertilizer, the combination of organic fertilizer and microbial inoculum had a significant effect on stem and leaf phosphorus, soil carbon, nitrogen, phosphorus and microbial biomass nitrogen (p<0.05), but had no significant effect on root nutrient. Among them, the combination of microbial inoculum and A1 organic fertilizer had the greatest impact on soil total phosphorus, increasing by 90.06%. Under the combination application of A2 organic fertilizer, the soil organic carbon and biomass nitrogen were affected most obviously, increasing by 104.60% and 71.95%, respectively. Under the combination application of A3 organic fertilizer, the total phosphorus in stem and leaf and soil total nitrogen were significantly affected. The total phosphorus in stem and leaf decreased by 183.96%, while soil total nitrogen increased by 29.14%. (2) Under the fertilization treatment, compared to the stem and leaf and microorganisms, the internal stability of S. flavescens root was weaker and more sensitive to exogenous nutrient inputs. (3) Under fertilization treatment, there was a strong coupling relationship between plant root nutrients and soil and soil microorganisms, so the nutrient balance of each component could be coordinated through root sensitivity to maintain plant stability.