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Development of a walleye cell line and use to study the effects of temperature on infection by viral haemorrhagic septicaemia virus group IVb |
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| Authors: | N T K Vo A W Bender L E J Lee J S Lumsden N Lorenzen B Dixon N C Bols |
| Affiliation: | 1. Department of Biology, University of Waterloo, Waterloo, ON, Canada;2. Faculty of Science, University of the Fraser Valley, Abbotsford, BC, Canada;3. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada;4. Department of Animal Sciences, Aarhus University, Aarhus, Denmark;5. Department of Biology, University of Waterloo, Waterloo, ON, Canada Correspondence N C Bols, Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada (e-mail: ncbols@uwaterloo.ca) |
| Abstract: | A cell line, WE-cfin11f, with a fibroblast-like morphology was developed from a walleye caudal fin and used to study the intersection of thermobiology of walleye, Sander vitreus (Mitchill), with the thermal requirements for replication of viral haemorrhagic septicaemia virus (VHSV) IVb. WE-cfin11f proliferated from 10 to 32 °C and endured as a monolayer for at least a week at 1–34 °C. WE-cfin11f adopted an epithelial shape and did not proliferate at 4 °C. Adding VHSV IVb to cultures at 4 and 14 °C but not 26 °C led to cytopathic effects (CPE) and virus production. At 4 °C, virus production developed more slowly, but Western blotting showed more N protein accumulation. Infecting monolayer cultures at 4 °C for 7 days and then shifting them to 26 °C resulted in the monolayers being broken in small areas by CPE, but with time at 26 °C, the monolayers were restored. These results suggest that at 26 °C, the VHSV IVb life cycle stages responsible for CPE can be completed, but the production of virus and the initiation of infections cannot be accomplished. |
| Keywords: | caudal fin fibroblasts thermobiology thermovirology VHSV walleye |