| 发根农杆菌对栝楼的遗传转化 |
| |
| 引用本文： | 宁志怨,董玲,陈静娴,廖华俊,江芹,张永娣.发根农杆菌对栝楼的遗传转化[J].安徽农业科学,2007,35(14):4142-4143. |
| |
| 作者姓名： | 宁志怨 董玲 陈静娴 廖华俊 江芹 张永娣 |
| |
| 作者单位： | 安徽省农业科学院园艺研究所,安徽,合肥,230031;潜山县农委栝楼办公室,安徽潜山,246300 |
| |
| 基金项目： | 鸣谢 发根农杆菌R1601,R1000,LBA9402由中国科学院植物研究所赵德修研究员提供,特此致谢. |
| |
| 摘 要： | 利用5种发根农杆菌1.2556、A4、LBA9402、R1000、R1601对栝楼组培苗茎段和叶片进行感染,诱导出毛状根,PCR检测结果表明,发根农杆菌Ri质粒的rolB基因已经整合到栝楼的基因组中.
|
| 关 键 词： | 发根农杆菌 栝楼 毛状根 PCR检测 |
| 文章编号： | 0517-6611（2007）14-04142-02 |
| 收稿时间： | 2007-02-06 |
| 修稿时间： | 2007-02-06 |
Genetic Transformation of Trichosanthes kirilowii Maxim by Agrobacterium rhizogenes |
| |
| NING Zhi-yuan et al.Genetic Transformation of Trichosanthes kirilowii Maxim by Agrobacterium rhizogenes[J].Journal of Anhui Agricultural Sciences,2007,35(14):4142-4143. |
| |
| Authors: | NING Zhi-yuan |
| |
| Institution: | Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031 |
| |
| Abstract: | Stem segments and leaves of Trichosanthes kirilowii Maxim were infected by five Agrobacterium rhizogenes(1.2556,A4,LBA9402,R1000 and R1601).Hairy root was induced.The result of PCR showed that rolB gene in Ri plasmid was integrated into the genome of Trichosanthes kirilowii Maxim. |
| |
| Keywords: | Agrobacterium rhizogenes Trichosanthes kirilowii Maxim Hairy root PCR detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
