| Bar基因转化草原1号苜蓿的研究 |
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| 引用本文： | 刘艳芝,王玉民,刘莉,隋松兵,李望丰,董英山. Bar基因转化草原1号苜蓿的研究[J]. 草地学报, 2004, 12(4): 273-275,280. DOI: 10.11733/j.issn.1007-0435.2004.04.004 |
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| 作者姓名： | 刘艳芝 王玉民 刘莉 隋松兵 李望丰 董英山 |
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| 作者单位： | 吉林省农业科学院生物技术研究中心, 吉林, 公主岭, 136100 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 利用农杆菌介导法将抗草丁膦的Bar基因导入草原1号苜蓿,经叶片筛选试验证实转基因植株对除草剂Basta具有抗性,通过多聚白酶连式反映检测,初步证实目的基因已转入到苜蓿基因组中.
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| 关 键 词： | 生物技术 苜蓿 农杆菌介导 遗传转化 |
| 文章编号： | 1007-0435(2004)04-0273-04 |
| 收稿时间： | 2003-09-10 |
Agrobacterium-Mediated Transformation of Alfalfa Variety,Caoyuan No.1,by Bar Gene |
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| LIU Yan-zhi,WANG Yu-min,LIU Li,SUI Song-bing,LI Wang-feng,DONG Ying-shan. Agrobacterium-Mediated Transformation of Alfalfa Variety,Caoyuan No.1,by Bar Gene[J]. Acta Agrestia Sinica, 2004, 12(4): 273-275,280. DOI: 10.11733/j.issn.1007-0435.2004.04.004 |
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| Authors: | LIU Yan-zhi WANG Yu-min LIU Li SUI Song-bing LI Wang-feng DONG Ying-shan |
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| Affiliation: | Biotechnology Research Center, Jilin Academy of Agri culture Sciences, Gongzhuling, Jilin Province, 136100, China |
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| Abstract: | Bar gene coding Glufosinate resistance is transformed into the alfalfa variety, Caoyuan No.1, mediated by Agrobacterium tumefaciens. The leaves of transgenic plants after being screened and tested are proved to be resistant to Glufosinate. Furthermore, PCR analysis shows that bar gene has been transformed into Alfalfa. |
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| Keywords: | Biotechnology Alfalfa Agrobacterium tumefaciens Genetic transformation |
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