蒲公英提取物对乳房炎病原菌的抑菌活性分析

为了研究蒲公英的有效抑菌成分,利用超声提取技术对蒲公英进行提取,通过大孔吸附树脂D101分离其活性部分。试管法试验表明40%乙醇洗脱部分含有酚类物质,90%乙醇洗脱部分含有黄酮类化合物。经药敏试验证明90%和40%的乙醇洗脱部分为蒲公英的主要抑菌部位,对奶牛隐性乳房炎主要病原菌金黄色葡萄球菌、大肠杆菌、无乳链球菌、停乳链球菌和乳房链球菌有明显的抑菌效果。

The Bacteriostasis of the Extracts from Herba taraxaci on Bacteria Derived from Bovine Hidden Mastitis

In order to investigate the effective antibacterial components of the Herba Taraxaci,the extraction of Herba Taraxaci(dandelion) was prepared by ultrasonic technique and different components of it were separated through macroporous adsorption resin D101.The test tube method was used to examine the bacteriostasis of each component.The results showed that phenolic composition was mainly existed in the fraction eluted with 40% of ethanol,and the flavonoids in the fraction eluted with 90% of ethanol.The susceptibility tests indicated that the fractions eluted with 90% and 40% ethanol were the chief antibacterial parts of dandelion,which had significant inhibitory efficacy on bacteria derived from bovine hidden mastitis,including S.aureas,E.coli,S.dysagalactiae,S.agalactiae and S.uberis.