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蒲公英提取物对乳房炎病原菌的抑菌活性分析
引用本文:宋毓民,李定刚,张鸯,张武岗,靳亚平,周乐,唐金宁. 蒲公英提取物对乳房炎病原菌的抑菌活性分析[J]. 西北农业学报, 2006, 15(4): 55-57
作者姓名:宋毓民  李定刚  张鸯  张武岗  靳亚平  周乐  唐金宁
作者单位:1. 西北农林科技大学动物科技学院,陕西杨凌,712100
2. 青岛康地恩实业有限公司,山东青岛,266061
3. 西北农林科技大学生命科学学院,陕西杨凌,712100
4. 山东省临沂市动植物园,山东临沂,276700
摘    要:为了研究蒲公英的有效抑菌成分,利用超声提取技术对蒲公英进行提取,通过大孔吸附树脂D101分离其活性部分。试管法试验表明40%乙醇洗脱部分含有酚类物质,90%乙醇洗脱部分含有黄酮类化合物。经药敏试验证明90%和40%的乙醇洗脱部分为蒲公英的主要抑菌部位,对奶牛隐性乳房炎主要病原菌金黄色葡萄球菌、大肠杆菌、无乳链球菌、停乳链球菌和乳房链球菌有明显的抑菌效果。

关 键 词:蒲公英  大孔吸附树脂  抑菌成分
文章编号:1004-1389(2006)04-0055-03
收稿时间:2005-08-22
修稿时间:2006-02-24

The Bacteriostasis of the Extracts from Herba taraxaci on Bacteria Derived from Bovine Hidden Mastitis
SONG Yu-min,LI Ding-gang,ZHANG Yang,ZHANG Wu-gang,JIN Ya-ping,ZHOU Le and TANG Jin-ming. The Bacteriostasis of the Extracts from Herba taraxaci on Bacteria Derived from Bovine Hidden Mastitis[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(4): 55-57
Authors:SONG Yu-min  LI Ding-gang  ZHANG Yang  ZHANG Wu-gang  JIN Ya-ping  ZHOU Le  TANG Jin-ming
Abstract:In order to investigate the effective antibacterial components of the Herba Taraxaci,the extraction of Herba Taraxaci(dandelion) was prepared by ultrasonic technique and different components of it were separated through macroporous adsorption resin D101.The test tube method was used to examine the bacteriostasis of each component.The results showed that phenolic composition was mainly existed in the fraction eluted with 40% of ethanol,and the flavonoids in the fraction eluted with 90% of ethanol.The susceptibility tests indicated that the fractions eluted with 90% and 40% ethanol were the chief antibacterial parts of dandelion,which had significant inhibitory efficacy on bacteria derived from bovine hidden mastitis,including S.aureas,E.coli,S.dysagalactiae,S.agalactiae and S.uberis.
Keywords:Herba taraxaci  The macroporous adsorption resin  Antibacterial component
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