| 142份甜高粱品种的分子身份证构建 |
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| 引用本文： | 王黎明,焦少杰,姜艳喜,严洪冬,苏德峰,孙广全.142份甜高粱品种的分子身份证构建[J].作物学报,2011,37(11):1975-1983. |
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| 作者姓名： | 王黎明 焦少杰 姜艳喜 严洪冬 苏德峰 孙广全 |
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| 作者单位： | 黑龙江省农业科学院作物育种研究所，黑龙江哈尔滨 150086 |
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| 基金项目： | 现代农业产业技术体系专项资金(nycytx-12-03-02); 黑龙江省科技攻关项目(GA09B101-5-12)项目资助 |
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| 摘 要： | 从分布在高粱染色体10个连锁群上的103对SSR引物中，筛选出41对多态性引物, 并用其扩增国内外142份甜高粱种质资源, 检测到189个多态性片段, 每对引物的等位基因数在2~11之间，平均为4.6个。引物位点的多态信息含量指数(PIC)变幅为0.089~0.850，平均为0.543。品种间特异指数差异较大，介于109.1~454.7之间，平均为189.0。结果表明, 根据引物的等位基因数确定11对引物(Xtxp329、Xtxp258、Xtxp113、Xtxp303、Xtxp61、Xtxp201、Xtxp14、Xtxp91、Xtxp47、Xtxp217和Xtxp67)组合, 并用于构建142份品种资源的分子身份证, 有效地区分了各品种。
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| 关 键 词： | 甜高粱 种质资源 分子身份证 |
| 收稿时间： | 2011-05-25 |
Establishment of Molecular Identity in 142 Sweet Sorghum Varieties |
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| WANG Li-Ming,JIAO Shao-Jie,JIANG Yan-Xi,YAN Hong-Dong,SU De-Feng, SUN Guang-Quan Crop Breeding Institute,Heilongjiang Academy of Agricultural Sciences,Harbin ,China.Establishment of Molecular Identity in 142 Sweet Sorghum Varieties[J].Acta Agronomica Sinica,2011,37(11):1975-1983. |
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| Authors: | WANG Li-Ming JIAO Shao-Jie JIANG Yan-Xi YAN Hong-Dong SU De-Feng SUN Guang-Quan Crop Breeding Institute Heilongjiang Academy of Agricultural Sciences Harbin China |
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| Institution: | Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| Abstract: | DNA identity (ID) is highly effective and efficient to distinguish crop varieties regardless of their similarities in phenotypes. To establish the DNA ID constitution in sweet sorghum, we screened 103 SSR primer pairs that covered 10 linkage groups in 10 varieties of the crop, and selected 41 primer pairs with clear polymorphic bands. These SSR primers were then amplified in 142 accessions of domestic and exotic sweet sorghum germplasm. A total of 189 polymorphic bands were obtained with 2-11 bands per prim... |
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| Keywords: | Sweet sorghum Germplasm resources Molecular identity |
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