| 栽培大豆和野生大豆的Glyma13g21630基因多样性分析 |
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| 引用本文： | 张乐,李英慧,刘章雄,邱丽娟.栽培大豆和野生大豆的Glyma13g21630基因多样性分析[J].作物学报,2011,37(10):1724-1734. |
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| 作者姓名： | 张乐 李英慧 刘章雄 邱丽娟 |
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| 作者单位： | 农作物基因资源与遗传改良国家重大科学工程 / 农业部作物种质资源利用重点开放实验室 / 中国农业科学院作物科学研究所，北京100081 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2010CB125903); 国际科技合作项目(2008DFA30550)资助 |
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| 摘 要： | 利用Sanger方法对49份野生大豆，46份地方品种和38份选育品种的Glyma13g21630基因测序，采用DNAStar、Mega、DNAsp和Tassel软件分析Glyma13g21630的单核苷酸多态性(SNP)位点在不同类型大豆群体的分布规律。结果表明，在133份供试种质中检测到多态性位点29个，包括22个SNP和7个InDel，多态性频率分别为1SNP/138 bp和1InDel/434 bp。Glyma13g21630基因序列中多态性位点的分布不均匀，其中内含子3和5为变异富集区，其他区域变异较小。单倍型分析表明，Glyma13g21630在野生大豆和栽培大豆中的多态性位点数目逐渐减少，分布范围也越来越窄；连锁不平衡分析表明，野生大豆的7个高频SNP位点中有42.86%处于极显著的连锁不平衡状态；Ka/Ks>1，说明野生大豆在向栽培大豆的进化中，某些位点受到正向选择，多态性显著降低。Glyma13g21630基因在大豆由野生向栽培驯化的过程中因正向选择作用而固定了有益变异，表现出瓶颈效应。
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| 关 键 词： | 大豆 Glyma13g21630 单核苷酸多态性 单倍型 |
| 收稿时间： | 2011-03-15 |
Gene Glyma13g21630 Diversity in Cultivated (G. max) and Wild (G. soja) Soy-beans |
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| ZHANG Le,LI Ying-Hui,LIU Zhang-Xiong, QIU Li-JuanNational Key Facility for Crop Gene Resources , Genetic Improvement / Key Laboratory of Germplasm Utilization,Ministry of Agriculture / Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing ,China.Gene Glyma13g21630 Diversity in Cultivated (G. max) and Wild (G. soja) Soy-beans[J].Acta Agronomica Sinica,2011,37(10):1724-1734. |
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| Authors: | ZHANG Le LI Ying-Hui LIU Zhang-Xiong QIU Li-JuanNational Key Facility for Crop Gene Resources Genetic Improvement / Key Laboratory of Germplasm Utilization Ministry of Agriculture / Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing China |
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| Institution: | National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Germplasm Utilization, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Glyma13g21630 from soybean is homologous with a gene related to leaf size from Arabidopisis thaliana, and it ex-perienced artifical selection during domestication in a small sample set. This paper aims at analyzing single nucleotide polymor-phism of Glyma13g21630 in a large sample set and providing the foundation for tracing back to soybean domestication and ana-lyzing the genetic basis of domesticated traits. According to Sanger method, PCR products of Glyma13g21630 from 49 wild soy-bean and 84 cultivars (... |
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| Keywords: | Soybean Glyma13g21630 Single nucleotide polymorphism Haplotype |
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