| 陆地棉质体转录活性因子基因GhPTAC的克隆及其耐盐性分析 |
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| 引用本文： | 周凯,叶武威,王俊娟,王德龙,樊保香,王帅. 陆地棉质体转录活性因子基因GhPTAC的克隆及其耐盐性分析[J]. 作物学报, 2011, 37(9): 1551-1558. DOI: 10.3724/SP.J.1006.2011.01551 |
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| 作者姓名： | 周凯 叶武威 王俊娟 王德龙 樊保香 王帅 |
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| 作者单位： | 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室 / 农业部棉花遗传改良重点实验室,河南安阳 455000 |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项(2008ZX08005-004); 国家“十一五”科技支撑计划项目(2006BAD13B04-1)资助 |
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| 摘 要： | 为了挖掘新的耐盐基因及调控途径,利用基因芯片技术及抑制性差减文库技术筛选到质体转录活性因子,通过RACE及RT-PCR技术克隆到该基因的cDNA全长,命名为GhPTAC。该cDNA全长1 564 bp,其中ORF 1 038 bp,推测编码345个氨基酸残基的多肽。生物信息学分析表明GhPTAC为拟南芥PTAC13同源基因,同源性60.6%。编码蛋白为转录活跃的染色体(TAC)的一个组分,参与叶绿体基因组转录终止/抗终止调节。Real-time PCR分析结果表明,GhPTAC受盐胁迫诱导上调表达,在耐盐材料中9806中表达水平明显高于盐敏感材料中S9612,这与芯片结果一致。
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| 关 键 词： | 陆地棉 盐胁迫 PTAC Real-time PCR |
| 收稿时间： | 2011-02-28 |
Cloning and Salt-tolerance Analysis of Gene Plastid Transcriptionally Active(GhPTAC) from Gossypium hirsutum L. |
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| ZHOU Kai,YE Wu-Wei,WANG Jun-Juan,WANG De-Long,FAN Bao-Xiang, WANG Shuai Cotton Research Institute,Chinese Academy of Agricultural Science/State Key Laboratory of Cotton Biology /Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry,Anyang ,China. Cloning and Salt-tolerance Analysis of Gene Plastid Transcriptionally Active(GhPTAC) from Gossypium hirsutum L.[J]. Acta Agronomica Sinica, 2011, 37(9): 1551-1558. DOI: 10.3724/SP.J.1006.2011.01551 |
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| Authors: | ZHOU Kai YE Wu-Wei WANG Jun-Juan WANG De-Long FAN Bao-Xiang WANG Shuai Cotton Research Institute Chinese Academy of Agricultural Science/State Key Laboratory of Cotton Biology /Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry Anyang China |
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| Affiliation: | Cotton Research Institute, Chinese Academy of Agricultural Science / State Key Laboratory of Cotton Biology / Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry, Anyang 455000, China |
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| Abstract: | To develop novel salt-tolerance genes and adjustment pathway,we screened out gene Plastid Transcriptionally Active named as GhPTAC based on salt-tolerance gene chips and salt resistance related SSH library.The result of RACE and RT-PCR showed that the cDNA full length was 1 564 bp,and ORF was 1 038 bp,which encoded 345 amino acid residues.Bioinformatics analysis showed that GhPTAC shared the identity of 60.6% with the homologous gene PTAC13 from Arabidopsis thaliana.As one part of Transcriptionally active c... |
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| Keywords: | Gossypium hirsutum Salt stress PTAC real-time PCR |
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