| 薜荔茎段的组织培养与植株再生技术 |
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| 引用本文： | 姜傲芳,田大伦,谭晓风,张党权,曾艳玲,杨伟. 薜荔茎段的组织培养与植株再生技术[J]. 中南林业科技大学学报(自然科学版), 2007, 27(3): 10-13 |
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| 作者姓名： | 姜傲芳 田大伦 谭晓风 张党权 曾艳玲 杨伟 |
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| 作者单位： | 中南林业科技大学经济林育种与栽培国家林业局重点实验室 湖南长沙410004(姜傲芳，谭晓风，张党权，曾艳玲，杨伟)，中南林业科技大学生态教研室 湖南长沙410004(田大伦) |
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| 基金项目： | 引进国际先进农业科技计划(948计划) |
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| 摘 要： | 以薜荔茎段为外植体,研究了其丛生芽诱导和快繁技术.实验结果表明,B5为最适合的基本培养基,根据正交试验结果,各激素对诱导丛生芽的作用大小为:6-BA>NAA>KT>IAA,诱导丛生芽的最佳培养基为:B5 1 mg/L NAA 0.1 mg/L IAA 1mg/L KT 2 mg/L 6-BA 蔗糖20 g/L 琼脂0.75%;生根培养基以B5 3 mg/L NAA 0.1 mg/L IAA 1 mg/L KT 2 mg/L6-BA 蔗糖20 g/L 琼脂0.75%为宜.
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| 关 键 词： | 薜荔 组织培养 快速繁殖 茎段 正交试验 |
| 文章编号： | 1673-923X（2007）03-0010-04 |
| 收稿时间： | 2007-03-27 |
| 修稿时间： | 2007-03-27 |
Tissue Culture of Stem Segment and Plantlet Regeneration of Ficus pumila L. |
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| JIANG Ao-fang,TIAN Da-lun,TAN Xiao-feng,ZHANG Dang-quan,ZENG Yan-ling,YANG Wei. Tissue Culture of Stem Segment and Plantlet Regeneration of Ficus pumila L.[J]. , 2007, 27(3): 10-13 |
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| Authors: | JIANG Ao-fang TIAN Da-lun TAN Xiao-feng ZHANG Dang-quan ZENG Yan-ling YANG Wei |
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| Affiliation: | 1. The Key Lab. of Non-wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; 2. Research Section of Ecology, Central South University of Forestry and Technology, Changsha 410004, Hunan, China |
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| Abstract: | In this study,we investigated the tissue culture and plantlet regeneration of Ficus pumila L.using its stem segment as the explants.The resultshows that the optimal basic medium suitable for Ficus pumila L.is the B5 medium.Based on the result of orthogonal experiment,the effect of various hormones on induction of clump buds is determined as follows: 6-BA > NAA > KT > IAA,and the optimum medium for inducing clump buds is B5 1 mg/L NAA 0.1 mg/L IAA 1 mg/L KT 2 mg/L 6-BA cane sugar 20 g/L agar 0.75%.For rooting,B5 3 mg/L NAA 0.1 mg/L IAA 1 mg/L KT 2 mg/L 6-BA cane sugar 20 g/L agar 0.75% has a better effect. |
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| Keywords: | Ficus pumila L. tissue culture rapid regeneration stem segment orthogonal experiment |
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