Use of a fast test to detect rotavirus in feces |
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Authors: | Otto P Elschner M Schulze P Prudlo J Schrader R |
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Institution: | Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterin?rmedizin, Jena. |
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Abstract: | The commercially available immunoassay "OnSite Rotavirus" was used for the detection of animal rotaviruses in 113 faecal samples. The sensitivity of the test was 88% and the specificity 96% compared with reference methods (EIA, EM). This test would detect approximately 4.4 x 10(6) to 1.8 x 10(7) virus particles per ml. The presence of virus could be demonstrated in fresh faecal samples from cattle, horses and pigs within a few minutes. The rotaviruses of group A were identified independently of the virus serotype. Further results and additional problems of using this test kit are described. |
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