| ELISA检测DLV虫苗免疫鸡抗体消长规律的研究 |
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| 引用本文： | 苏华炜,邹晖.ELISA检测DLV虫苗免疫鸡抗体消长规律的研究[J].中国兽医寄生虫病,1995,3(3):20-23. |
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| 作者姓名： | 苏华炜 邹晖 |
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| 作者单位： | 中国农科院上海家畜寄生虫病研究所 |
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| 摘 要： | 将柔嫩、毒害、巨型艾美耳球虫的孢子化卵囊制成可溶性抗原，用酶联免疫吸附试验检测鸡球虫双重致弱多凤苗虫苗、为柔嫩、毒害、巨型艾美耳球虫三价苗免疫鸡血清抗球虫抗体的消长规律。
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| 关 键 词： | ELISA DLV球虫苗 鸡病 抗体 检测 |
STUDIES ON DETECTION OF SERUM ANTIBODIES TITER IN DOUBLY ATTENUATED VACCINE(DLV)-IMMUNES CHICKENS BY ELISA |
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| Sa Huawei,Zhou Hui,Wu Zhaomin.STUDIES ON DETECTION OF SERUM ANTIBODIES TITER IN DOUBLY ATTENUATED VACCINE(DLV)-IMMUNES CHICKENS BY ELISA[J].Chinese Journal of Veterinary Parasitology,1995,3(3):20-23. |
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| Authors: | Sa Huawei Zhou Hui Wu Zhaomin |
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| Abstract: | Soluble antigens prepared from sporulated oocysts of E.tenella,B.necatrix and E.maxima were used for ELISA to investigate antibodies titer in sera of chickens immuned with doubly attenuated vaccine (DLV) at 3,8 and 16 days age.ELISA values increased rapidly after day 4 post immunization and reached the maximus levels after day 7 post immunization against both E. tenella and E.necatrix.ELISA values against E.maxima increased rapidly after immunization and reached the maximus level after day 6 post immunization.ELISA values would keep at high levels to 50 days old at least.All these revealed that the DLV was safe,stable and effective. |
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| Keywords: | soluble oocyst antigens ELISA DLV |
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