| 电子束辐射诱变及克隆选择培育好好芭抗寒无性系的研究 |
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| 引用本文： | 张根发,王舒静,胡伯继,张桂芳,石小明,王纯.电子束辐射诱变及克隆选择培育好好芭抗寒无性系的研究[J].中国油料作物学报,2003,25(4):56-59. |
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| 作者姓名： | 张根发 王舒静 胡伯继 张桂芳 石小明 王纯 |
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| 作者单位： | 1. 北京师范大学生命科学学院,北京100875
2. 河南省土壤肥料站,河南,郑州450003 |
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| 基金项目： | 农业部948项目;北京市自然科学基金项目(5982015) |
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| 摘 要： | 利用电子辐射处理好好芭试管苗，从改变基因型入手提高其抗寒力，以获得稳定遗传的好好芭株系。研究表明150GY的辐射量是诱发好好芭发生变异的最佳剂量，结合生化标记，抗寒相关的酶类——淀粉酶和过氧化物酶活性分析，人工模拟低温实验的克隆检测，筛选出了具有较高抗寒能力的好好芭株系，这些株系通过无性系繁殖可用于抗寒育种。
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| 关 键 词： | 电子束辐射诱变 克隆筛选 培育技术 好好芭 抗寒无性系 生物学效应 |
| 文章编号： | 1007-9084(2003)04-0056-04 |
| 修稿时间： | 2003年4月16日 |
Primary study on cold tolerant lines of Jojoba by using electron beam irradiation and clone selection |
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| ZHANG Gen--fa,WANG Shu--jing,HU Bo--ji,ZHANG Gui--fang,SHI Xiao--ming,WANG Chun College of Life Sciences,Beijing Normal University,Beijing ,China The Station of Soil and Fertilization of Henan Province,Zhengzhou ,China.Primary study on cold tolerant lines of Jojoba by using electron beam irradiation and clone selection[J].Chinese Journal of Oil Crop Sciences,2003,25(4):56-59. |
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| Authors: | ZHANG Gen--fa WANG Shu--jing HU Bo--ji ZHANG Gui--fang SHI Xiao--ming WANG Chun College of Life Sciences Beijing Normal University Beijing China The Station of Soil and Fertilization of Henan Province Zhengzhou China |
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| Institution: | ZHANG Gen--fa,WANG Shu--jing,HU Bo--ji,ZHANG Gui--fang,SHI Xiao--ming,WANG Chun College of Life Sciences,Beijing Normal University,Beijing 100875,China The Station of Soil and Fertilization of Henan Province,Zhengzhou 450003,China |
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| Abstract: | The electronic beam irradiation method was used to induce Jojoba somatic clone mutagensis for se-
lecting cold resistance clones after the exam of cold test experiment. Those clones with high level cold resistance
and stable heredity were selected. The results indicated that radiation of electron beam at 150Gy was the best dose
to induce Jojoba clones mutagensis. This dose was used to induce different clones and select cold resistant clones by
the experiment of frozen temperature treatments. A few of high cold tolerant clones were obtained in the research,
which will be used for further breeding of cold tolerance lines in Jojoba. |
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| Keywords: | Jojoba Mutagenic effect Electron beam Cold tolerance Clone selection |
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