| 猪肿瘤抑制基因候选5的克隆与序列分析 |
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| 引用本文： | 张志强,杨国宇,王伟杰,韩立强,王静,武宇晓,臧猛,台玉磊. 猪肿瘤抑制基因候选5的克隆与序列分析[J]. 河南农业科学, 2009, 0(1) |
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| 作者姓名： | 张志强 杨国宇 王伟杰 韩立强 王静 武宇晓 臧猛 台玉磊 |
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| 作者单位： | 河南农业大学,动物生理生化实验室,河南,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目 |
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| 摘 要： | 基于电子延伸序列,克隆了仔猪肿瘤抑制基因候选5(TUSC5),并初步分析其组织分布情况;获得了TUSC5基因的全长序列636bp,包括开放阅读框架(ORF)534bp,编码177个氨基酸。克隆的仔猪TUSC5基因与人、小鼠、大鼠的TUSC5基因序列的同源性分别为83.5%,82.4%,82.6%,推测的氨基酸序列同源性分别为71.2%,77.5%,78%,成功克隆了猪TUSC5基因。
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| 关 键 词： | 猪 TUSC5 克隆 序列分析 |
cDNA Cloning and Sequence Analysis of the Gene of Porcine Tumor Suppressor Candidate 5 |
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| ZHANG Zhi-qiang,YANG Guo-yu,WANG Wei-jie,HAN Li-qiang,WANG Jing,WU Yu-xiao,ZANG Meng,TAI Yu-lei. cDNA Cloning and Sequence Analysis of the Gene of Porcine Tumor Suppressor Candidate 5[J]. Journal of Henan Agricultural Sciences, 2009, 0(1) |
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| Authors: | ZHANG Zhi-qiang YANG Guo-yu WANG Wei-jie HAN Li-qiang WANG Jing WU Yu-xiao ZANG Meng TAI Yu-lei |
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| Affiliation: | Lab of Animal Physiology & Biochemistry;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | In this study,the coding sequence of porcine tumor suppressor candidate 5(TUSC5)were amplified based on the in silico sequence information. The result showed that the full length of TUSC5 was 636 bp and the open reading frame was 534 bp long. The predicted protein-coding sequence was 177 amino acid residues. Identity analysis showed that the nucleotide sequence of porcine TUSC5 shares 83.5%,82.4% and 82.6% with that of human,mouse and rat,respectively. The deduced protein sequence shares 71.2%,77.5%,and 78%... |
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| Keywords: | TUSC5 |
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