应用SHIME模型研究肠道微生物对13种农产品中 腐霉利生物可给性的影响

应用模拟人体胃肠消化的SHIME（simulator of the human intestinal microbial ecosystem）体外方法评价肠道菌群对不同农产品中腐霉利生物可给性的作用规律，为正确评价农产品中农药残留健康风险提供理论依据。结果表明：肠道菌群可使葡萄等13种农产品中腐霉利生物可给性降低30.3% ~ 97.2%，其中以葡萄和大豆中供试农药残留生物可给性的影响较为显著；膳食成分可增强肠道菌群对腐霉利生物可给性的影响，菊粉作用下可使肠道菌群对葡萄中腐霉利生物可给性降低39.6%。大肠杆菌是影响腐霉利生物可给性的主要优势菌，36 h时可使葡萄中腐霉利生物可给性显著降低33.9%。

Effects of gut microflora on procymidone bioaccessibility in 13 kinds of agricultural products by SHIME mode

The simulator of the human intestinal microbial ecosystem (SHIME) can be used in vitro gastrointestinal methods to determine the effects of gut microbiota on the procymidone bioaccessibility in different agricultural products, which can provide a theoretical basis for the reliable evaluation of potential health risks of pesticide residues in agricultural products. The results showed that gut microbiota could reduce the procymidone bioaccessibility by 30.3% - 97.2% in 13 agricultural products. Among the tested products, the pesticide residues bioaccessibility in grapes and soybeans were most significantly affected. Furthermore, dietary ingredients could enhance such kind of effect, for example, the addition of inulin could reduce the procymidone bioaccessibility in grapes by 39.6%. Otherwise, Escherichia coli was the superior bacteria of effects on procymidone bioaccessibility, which could result in a 33.9% decrease on the procymidone bioaccessibility in grapes after action for 36 h.