胶体金法与酶联免疫吸附测定法检测猪瘟抗体的比较

为比较胶体金法与ELISA法检测猪瘟抗体的符合率,以了解胶体金法的特异性与敏感度,用胶体金法和ELISA法平行检测受检猪血清标本猪瘟抗体滴度.结果表明,胶体金法和ELISA法检测猪瘟抗体的结果符合率为86.6％,胶体金法较ELISA法敏感度、特异度低,用胶体金法检测猪瘟抗体滴度存在一定程度的漏检率及误诊率,只能初步筛查猪瘟抗体.具有一定技术力量的县级兽医实验室使用ELISA法检测猪血样的猪瘟抗体滴度更为合适,对于胶体金法筛查阴性的猪血样,建议用ELISA法复检以防漏检.

Comparison of Colloidal Gold and ELISA on Swine Fever Antibody Detection

To study the specificity and sensitivity of colloidal gold method, we compared the detective coincidence rate of classical swine fever antibody（Anti-CSFV） between colloidal gold and ELISA. The Anti-CSFV titer of swine serum samples were tested by both colloidal gold and ELISA. The results showed that detective coincidence rate of Anti-CSFV were both 86. 6%, colloidal gold had missing detection and misdiagnose （false negative, false positive）. Compared with ELISA, colloidal gold has lowly specificity and sensitivity and the detection of Anti-CSFV titer has missing detection rate and misdiagnose rate, it can only be applied to preliminary screening of Anti-CSFV. ELISA is more appropriate to county level veterinary laboratories with strength technical for the detection of Anti-CSFV titer of swine serum samples. We suggest to use ELISA do redetect when colloidal gold had negative results to the samples.