桔小实蝇5个地理种群的SSR遗传多态性分析

桔小实蝇Bactrocera(Bactrocera)dorsalis(Hendel)是一种为害多种水果、瓜类、茄类蔬菜的检疫性害虫,食性杂、寄主范围广,直接威胁水果和蔬菜的安全生产。运用改进的CTAB法提取单头实蝇的基因组DNA,形态鉴定及特异性引物BD1/BD2PCR鉴定桔小实蝇,用7对微卫星标记(SSR)引物对采自中国福建、海南、广东、云南和四川5省的桔小实蝇种群间的遗传关系进行初步分析,DPS聚类分析结果显示,福建桔小实蝇与其他几个地区的桔小实蝇的差异性较大,达到0.86,另外4省之间遗传距离较近。

Microsatellite markers for genetic polymorphism in five geographic population of the Bactrocera dorsalis (Hendel)

The fruits flies Bactrocera （Bactrocera） dorsalis （Hendel） is one of the major economically important and quarantine insects in Asia. Genomic DNA of the sampled populations came from Fujian, Hainan, Guangdong, Yunnan and Sichuan,was extracted from thoracic tissue of individual adult ？ies by CTAB method and amplified with the polymerase chain reaction. By using microsatellite markers （SSR）, the fingerprint of five geographic population was investigated. With good reproducibility and high polymorphic, seven microsatellite primers were utilized in this study to assess the genetic polymorphism of orient fruit fly primarily. The results indicated that genetic differentiation exists among the five populations.