苹果AFL1基因原核表达载体的构建及其在大肠杆菌中的表达 |
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引用本文: | 张传义,孟玉平,孙海峰,曹秋芬. 苹果AFL1基因原核表达载体的构建及其在大肠杆菌中的表达[J]. 山西农业科学, 2008, 36(7) |
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作者姓名: | 张传义 孟玉平 孙海峰 曹秋芬 |
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作者单位: | 山西省农业生物技术研究中心,山西,太原,030031;山西省农业生物技术研究中心,山西,太原,030031;山西大学化学化工学院,山西,太原,030006 |
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基金项目: | 山西省科技攻关项目 , 山西省农业科学院高新技术项目 |
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摘 要: | AFL1基因是苹果控制花发育的关键基因之一。在已得到AFL1 cDNA基因的基础上,将AFL1克隆到原核表达载体pET28b中,获得了重组表达质粒pET-AFL1,并将此重组质粒转化到大肠杆菌菌株BL21中,经IPTG诱导表达、SDS-PAGE电泳分析,结果显示,AFL1基因在大肠杆菌中成功表达,表达的AFL1融合蛋白分子量大约为53 kD。
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关 键 词: | AFL1 原核表达 表达载体构建 SDS-PAGE |
The Construction of a Prokaryotic Expression Vector of Apple AFL1 and Its Expression in E.coli |
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Abstract: | AFL1 is one of key genes controlling flower development in apple.In this study,a prokaryotic expression vector pET-AFL1 was constructed and transformed into the competent cells of E.coli BL21 after having cloned AFL1 in apple.The transformed cell was induced with IPTG and expressed products were analyzed by SDS-PAGE, showing that AFL1 was expressed in E.coli successfully and the molecule weight of expressed fusion protein was about 53KD. |
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Keywords: | AFL1 Prokaryotic expression Construction of expression vector SDS-PAGE |
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