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| 莱航鸡rDNA重复基因家族的克隆 | |
| 引用本文： | 唐冬生,李芳,张勇,蒋泓,胡大林,张细权,李月琴,周天鸿. 莱航鸡rDNA重复基因家族的克隆[J]. 中国家禽, 2007, 29(3): 13-16 |
| 作者姓名： | 唐冬生 李芳 张勇 蒋泓 胡大林 张细权 李月琴 周天鸿 |
| 作者单位： | 1. 佛山大学,广东佛山,528000;暨南大学生命科学与技术学院,广东广州,510632 2. 佛山大学,广东佛山,528000 3. 华南农业大学动物科学学院,广东广州,510642 4. 暨南大学生命科学与技术学院,广东广州,510632 |
| 基金项目： | 国家自然科学基金;广东省自然科学基金;广东省科技攻关计划 |
| 摘 要： | 动物rDNA基因是一种GC含量较高、结构复杂的重复序列。通过结合生物信息学技术,经反复摸索后选用LAPCR法扩增莱航鸡rDNA基因重复序列,经测序鉴定最终克隆了莱航鸡的3个rDNA基因及其2个间隔序列。研究对克隆复杂DNA序列时引物设计的特别规则、反应体系的改进、DNA聚合酶的选用、循环参数的调整等进行了探索。 |
| 关 键 词： | rDNA基因 基因克隆 莱航鸡 |
| 修稿时间： | 2006-08-022006-11-27 |
Cloning on Complicated Repetitive Sequences of rDNA Genes Family in Leghorn Chicken |
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| Tang Dongsheng,Li Fang,Zhang Yong,Jiang Hong,Hu Dalin,Zhang Xiquan,Li Yueqin,Zhou Tianhong. Cloning on Complicated Repetitive Sequences of rDNA Genes Family in Leghorn Chicken[J]. China Poultry, 2007, 29(3): 13-16 | |
| Authors: | Tang Dongsheng Li Fang Zhang Yong Jiang Hong Hu Dalin Zhang Xiquan Li Yueqin Zhou Tianhong |
| Affiliation: | 1.Foshan University,Foshan,Guangdong 528000; 2.College of Life Science and Technology,Jinan University,Guangzhou,Guangdong 510632; 3.College of Animal Science,Southern China Agricultural University,Guangzhou,Guangdong 510642 |
| Abstract: | The rDNA genes in animals are the kind of repetitive sequences with high GC content and complicated structure. Combining the bioinformatics technique,the repetitive DNA sequences of rDNA family of Leghorn chicken were gained. The LA PCR method with LA PCR Taq DNA polymerase and GC buffer was used to amplify the complicated repetitive sequences of rDNA genes in Leghorn chicken after repeat experiment. Furthermore,the PCR products were cloned into pMD18-Simple T cloning plasmid. After sequencing identification three genes and two internal transcribed spacers (ITS1 and ITS2) of rDNA had been cloned. The measures for cloning the complicated DNA sequences such as the special rules of primer design,the improvement of reaction system,the selection of DNA polymerase and the adjustment of cycle parameter had been explored in this study. |
| Keywords: | rDNA genes gene cloning Leghorn chicken |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |