ACTINOMYCIN: INHIBITION OF CORTISONE-INDUCED SYNTHESIS OF HEPATIC GLUCONEOGENIC ENZYMES

The cortisone-induced de novo synthesis of liver glucose-6-phosphatase, fructose-1, 6-diphosphatase, aldolase, and lactic dehydrogenase was prevented by injections of Actinomycin D in the rat. In the in vitro assay systems, addition of actinomycin exerted no effect on the enzyme activities examined. The evidence favors the concept that the increased gluconeogenesis induced by corticoids entails at a certain stage an increased rate in the synthesis of gluconeogenic enzymes.