| 茶树根际土壤真菌ALF-1(Neurosporasp.)耐酸铝基因片段克隆 |
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| 引用本文： | 陆建良,梁月荣,吴颖,黄福平.茶树根际土壤真菌ALF-1(Neurosporasp.)耐酸铝基因片段克隆[J].茶叶科学,2004,24(1):41-44. |
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| 作者姓名： | 陆建良 梁月荣 吴颖 黄福平 |
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| 作者单位： | 浙江大学茶叶研究所,浙江,杭州,310029 |
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| 基金项目： | 博士点基金项目2000033539，国家基金项目30170591 |
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| 摘 要： | 采用rt-PCR克隆了茶树根际土壤耐酸铝真菌ALF-1(Neurospora sp.)与耐酸铝相关的基因片段,该片段由ALF-1在酸铝诱导条件才表达。序列分析后获得359 bp的可读片段,最大开放阅读框为260 bp,位于第100-359 bp之间,编码86个氨基酸残基。BLAST分析结果表明,该片段与Arabidopsis thaliana (thale cress)的耐酸铝相关基因序列同源性为50%。
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| 关 键 词： | 耐酸铝真菌 耐酸铝基因 克隆 |
| 文章编号： | 1000-369X(2004)01-041-03 |
| 修稿时间： | 2003年10月24 |
Cloning of Aluminum-tolerant Associated Gene from Fungus ALF-1(Neurospora sp.) in Rhizospheric Soil of Tea Plant |
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| LU Jian-liang,LIANG Yue-rong,WU Ying,HUANG Fu-ping.Cloning of Aluminum-tolerant Associated Gene from Fungus ALF-1(Neurospora sp.) in Rhizospheric Soil of Tea Plant[J].Journal of Tea Science,2004,24(1):41-44. |
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| Authors: | LU Jian-liang LIANG Yue-rong WU Ying HUANG Fu-ping |
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| Abstract: | Aluminum-tolerant associated gene of fungus ALF-1(Neurospora sp.) in rhizospheric soil of tea plant was cloned using rt-PCR technique. The gene expression was induced by aluminum treatment, and its sequence was 359 base pair (bp). The opening reading frame (ORF) was 260bp which situated from 100th to 359thbp, and encoded 86 amino acid residues. BLAST analysis showed that the gene sequence obtained was 50% identity to that of Arabidopsis thaliana (thale cress). |
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| Keywords: | Aluminum-tolerant fungus Aluminum-tolerant gene Cloning |
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