非淀粉多糖酶对蛋白酶体外酶解棉籽粕效果的影响研究

采用二步酶解法,先单独使用纤维素酶和果胶酶或二者合用,再用中性蛋白酶(中性蛋白酶500U/g酶解时间5h)酶解棉籽粕,以还原糖生成量、蛋白质水解度(degreeofhydrolysis,DH)和三氯乙酸氮溶指数(trichloroaceticacid-nitrogen solution index,TCA-NSI)为标识,研究NSP酶水平对还原糖生成量、DH和TCA-NSI的影响。结果为:在先用非淀粉多糖(non-starch polysaccharides,NSP)酶水解,然后用中性蛋白酶500U/g水解5h时,单独使用纤维素酶和果胶酶均可提高还原糖生成量、DH和TCA-NSI,适宜水平分别为:纤维素酶350U/g,果胶酶250U/g;同时使用纤维素酶和果胶酶,对提高还原糖生成量、DH具有协同效应,适宜水平为:纤维素酶350U/g和果胶酶250U/g。

Study on the effect of Non-starch polysaccharides to Hydrolyzation of Neutrase to Cottonseed Meal in Vitro

By adopting two-step enzymatic hydrolysis methods in which the cottonseed meal( CSM ) was hydrolyzed first by cellulase or/and pectinase for 5h and then hydrolyzed by neutrase( 500 U/g CSM) for 5 h,A serial trials were conducted to study the effect of cellulase or/and pectinase on the the production of reducing sugar ,degree of hydrolysis( DH ) and trichloroacetic acid-nitrogen solution index.( TCA-NSI ).The results showed that the cellulase or/and pectinase increased the production of reducing sugar ,DH and TCA-NSI.The optical cellulase or/and pectinase were 350U/g CSM or/and 250U/g CSM.there are synergetic effect between cellulase and pectinase which increased the production of reducing sugar.