“凤丹白”胚离体培养和植株再生研究

摘　要:以牡丹品种“凤丹白”的胚为外植体,研究了层积时间、基本培养基、植物激素等因素对胚愈伤组织的诱导及不定芽分化的影响。结果表明:40d层积时间对胚萌发的效果最好，MS+Ca2++2 mg•Ｌ-12,4-D+30ｇ•Ｌ-1蔗糖是胚诱导愈伤组织的最佳培养基，诱导率为85%；MS+ Ca2++1.0 mg•Ｌ-16-BA+0.5 mg•Ｌ-12,4-D+20ｇ•Ｌ-1。蔗糖是诱导不定芽发生的最佳培养基；高约1.5cm的不定芽转入生根培养基,生根率可达80%。

'Fengdanbai'Embryo Culture and Plantlet Regeneration of Peony

Abstract: ‘Fengdan’embryos were used as initial esxplants, the effect of the following factors on induction callus and differentiation adventitious buds:basal medium,plant hormone and stacked volume time were studied .Results indicated that the optimal time for embryos germination was 40d; the optimal medium for embryos induction callus was ＭＳ+Ca2++2 mg•L-1 2,4-D+30ｇ•L-1sucrose,the induction frequency was 85%;The optimal medum for differentiation adventitious buds was MS+ Ca2++1.0 mg•L-16-BA+0.5 mg•L-12,4-D+20ｇ•L-1.sucrose.Shoots with 1.5cm high were transferred to rooting medium and rooting frequency reached 80%.