| 我国部分省区HP-PRRSV分离株ORF5基因遗传变异分析 |
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| 引用本文： | 李平,吴发兴,朱紫祥,高许雷,张志,刘爽,张燕霞,李晓成,阿合买提. 我国部分省区HP-PRRSV分离株ORF5基因遗传变异分析[J]. 动物医学进展, 2009, 30(9): 1-6 |
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| 作者姓名： | 李平 吴发兴 朱紫祥 高许雷 张志 刘爽 张燕霞 李晓成 阿合买提 |
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| 作者单位： | 新疆农业大学动物医学学院,新疆乌鲁木齐,830052;中国动物卫生与流行病学中心,山东青岛,266032;中国动物卫生与流行病学中心,山东青岛,266032;中国动物卫生与流行病学中心,山东青岛,266032;西北农林科技大学,陕西杨陵,712100;中国动物卫生与流行病学中心,山东青岛,266032;青岛农业大学,山东青岛,266109;新疆农业大学动物医学学院,新疆乌鲁木齐,830052 |
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| 基金项目： | 十一五科技支撑计划项目 |
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| 摘 要： | 参考GenBank发表的猪繁殖与呼吸综合征病毒(PRRSV ) JXA1的ORF5基因序列,设计并合成了一对引物,对分离自云南、浙江、山东、辽宁、黑龙江、天津、湖南等7省12株PRRSV分离株进行RT-PCR扩增,获得约773 bp的DNA片段,将其分别克隆至pGEM-T Easy载体中,并进行测序.应用DNAStar软件分析序列,并与VR-2332、CH-la、MLV、LV、BJ4、HB1、HuB2、JXA1等毒株ORF5序列进行比较,结果表明,分离株间的核苷酸同源性为91.9%～100%,氨基酸同源性为90.1%～100%;在美洲株遗传关系上又分为明显的2个群:12株分离株与HB1、HuB2、JXA1、CH-la亲缘关系比较近,处于一个亚群;VR-2332、MLV、BJ4处于另一个亚群.说明高致病PRRSV为我国PRRS主要流行病毒.
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 遗传进化树 变异 |
Genetic Analysis of ORF5 Gene Sequences of Highly Pathogenic Porcine reproductive and respiratory syndrome virus in Some Provinces of China |
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| LI Ping,WU Fa-xing,ZHU Zi-xiang,GAO Xu-lei,ZHANG Zhi,LIU Shuang,ZHANG Yan-xia,LI Xiao-cheng,AHE Mai-ti. Genetic Analysis of ORF5 Gene Sequences of Highly Pathogenic Porcine reproductive and respiratory syndrome virus in Some Provinces of China[J]. Progress In Veterinary Medicine, 2009, 30(9): 1-6 |
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| Authors: | LI Ping WU Fa-xing ZHU Zi-xiang GAO Xu-lei ZHANG Zhi LIU Shuang ZHANG Yan-xia LI Xiao-cheng AHE Mai-ti |
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| Affiliation: | LI Ping1,2,WU Fa-xing2,ZHU Zi-xiang 2,3,GAO Xu-lei2,4,ZHANG Zhi2,LIU Shuang2,ZHANG Yan-xia2,LI Xiao-cheng2,AHE Mai-ti1(1.College of Veterinary Medicine,Xingjiang Agricultural University,Urumqi,Xinjiang,830052,China,2.China Animal Health and Epidemiology Center,Qingdao,Shandong,266032,3.Northwest A & F University,Yangling,Shaanxi,712100,4.Qingdao Agricultural University,266109,China) |
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| Abstract: | A pair of primers were designed according to the sequence of JXA1 strain of porcine reproductive and respiratory syndrome virus published in the GenBank for RT-PCR amplification of 12 PRRSV isolates from 7 provinces of Yunnan,Zhejiang,Shandong,Liaoning,Heilongjiang,Tianjing and Hunan in China.The DNA fragments of about 773 bp were obtained and cloned into PGEM-T Easy vectors and sequenced.The sequences of ORF5 genes were analysed using DNAStar and compared with the ORF5 genes of VR-2332,CH-la,MLV,LV,BJ4,HB1... |
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| Keywords: | Porcine reproductive and respiratory syndrome virus ORF5 gene phylogenetic tree variation |
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