| 百合品种IRAP指纹图谱构建 |
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| 引用本文： | 赵福宽,张建京,夏婧,范莹莹,张克. 百合品种IRAP指纹图谱构建[J]. 中国园艺文摘, 2010, 26(12): 5-6,36 |
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| 作者姓名： | 赵福宽 张建京 夏婧 范莹莹 张克 |
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| 作者单位： | [1]北京农学院生物技术系,北京102206 [2]北京农学院园林系,北京102206 |
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| 摘 要： | IRAP（inter—retrotransposon amplified polymorphism）是一种基于反转录转座子的分子标记技术。文章参考烟草中的反转录转座子Tnt1的LTR保守区域设计引物，对52个百合品种进行IRAP扩增，共扩增出165条多态性带，每个品种的扩增位点数介于0～8之间，据构建的指纹图谱可将供试百合品种完全区分开。
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| 关 键 词： | 反转录转座子 IRAP 百合 指纹图谱 |
Construction of Fingerprinting Map for Lily by IRAP Molecular Marker |
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| ZHAO Fu-kuan,ZHANG Jian-jing,XIA Jing,FAN Ying-ying,Zhang Ke. Construction of Fingerprinting Map for Lily by IRAP Molecular Marker[J]. Chinese Horticulture Abstracts, 2010, 26(12): 5-6,36 |
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| Authors: | ZHAO Fu-kuan ZHANG Jian-jing XIA Jing FAN Ying-ying Zhang Ke |
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| Affiliation: | ZHAO Fu-kuan,ZHANG Jian-jing, XIA Jing ,FAN Ying-ying, Zhang Ke |
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| Abstract: | IRAP （inter retrotransposon amplified polymorphism） is a kind of molecular markers based on retrotransposon. Primer was designed based on LTR reserve region of retrotransposon Tntl in tobacco. 32 varieties of lily were used as experimental material for IRAP amplification. Totally 163 polymorphic bands were amplified, and amplified loci quantity were 0-8 for each variety. All the 32 lily varieties could be identified by the fingerprinting map. |
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| Keywords: | retrotransposon IRAP Lily fingerprinting map |
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