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| SARS-CoVS基因重组质粒的构建与瞬时表达 | |
| 引用本文： | 王陆宝,冉多良,王红霞,孟庆文,徐佳,吴东来. SARS-CoVS基因重组质粒的构建与瞬时表达[J]. 新疆农业大学学报, 2005, 28(4): 50-52 |
| 作者姓名： | 王陆宝 冉多良 王红霞 孟庆文 徐佳 吴东来 |
| 作者单位： | 1. 中国农业科学院,哈尔滨兽医研究所,哈尔滨,150001;新疆农业大学,动物医学院,乌鲁木齐,830052 2. 新疆农业大学,动物医学院,乌鲁木齐,830052 3. 中国农业科学院,哈尔滨兽医研究所,哈尔滨,150001 |
| 基金项目： | 哈尔滨兽医研究所所长基金资助 |
| 摘 要： | 根据GenBank中登录的SARS-COV（BJ01株）基因序列设计引物，采用反转录聚合酶链反应（RT-PCR）方法从SARS病毒中扩增得到约3768bp的片段，将其克隆到真核表达质粒pVAX1，鉴定正确后．命名为pVAX1／S。体外转染Hela细胞．间接免疫荧光鉴定S蛋白的表达。构建的重组质粒经酶切及测序鉴定．开放阅读框正确；间接免疫荧光方法鉴定该重组质粒能在Hela细胞中表达。 |
| 关 键 词： | 传染性非典型肺炎 S基因 瞬时表达 |
| 文章编号： | 1007-8614(2005)04-0050-03 |
| 修稿时间： | 2005-11-03 |
Construction and Transient Expression of SARS-CoV S Gene Recombinant Plasmid |
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| WANG Lu-bao,RAN Duo-liang,WANG Hong-xia,MENG Qing-wen,XU Jia,WU Dong-lai. Construction and Transient Expression of SARS-CoV S Gene Recombinant Plasmid[J]. Journal of Xinjiang Agricultural University, 2005, 28(4): 50-52 | |
| Authors: | WANG Lu-bao RAN Duo-liang WANG Hong-xia MENG Qing-wen XU Jia WU Dong-lai |
| Affiliation: | WANG Lu-bao~ |
| Abstract: | According to the data in Genebank,the specific primers of the SARS-CoV S gene was designed.The S gene was amplified from SARS-CoV by RT-PCR.Then it was inserted into the eukaryotic expression vector pVAX1,named pVAX1/S.After transfecting Hela cell with pVAX1/S,the expression of S protein was detected with indirect immunofluorescent method.The result indicated that recombinant plasmid of (pVAX1/S) was constructed successfully.Expression of S protein was detected by indirect immunofluorescent method. |
| Keywords: | SARS-COV Spike Gene transient expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |