春蚕豆试管苗生根培养研究

目的]为利用组织培养技术进行蚕豆种子快繁提供理论依据。方法]以青海9号蚕豆为供试材料,研究了不同种类的生长调节剂、BA与生长调节剂、高浓度IBA和基部遮光法对青海9号试管苗的生根作用。处理30 d后,统计生根株数、每株生根数和根长,计算生根率。结果]单一使用IAA时生根率为0,单一的IBA在1.0 mg/L浓度下的效果最好,生根率为53.20%,平均每株生根数为3.39条,平均根长2.84 cm。在3种不同浓度的IAA和BA组合作用下,生根率均为0;3种不同浓度的IBA和BA组合作用下,以0.04 mg/L BA+1.4 mg/L IBA处理的效果最好,生根率为73.10%。100 mg/L的IBA效果最好,生根率50.32%,平均生根数3.21条,平均根长为2.26cm。基部遮光法处理的生根效果不如黑暗培养的效果好。结论]0.04 mg/L BA+1.4 mg/L IBA处理的生根率最高。

Study on Rooting and Culture of Spring Vicia faba L.Plantlet

Objective] The study was to provide the theoretical basis for the seed rapid propagation of Vicia faba L. by using the technique of tissue culture.Method] With the Qinghai 9 as the tested materials,the rooting effects of the different kinds of growth regulators,BA and the growth regulators,IBA with high concn.and basal part shading on plantlet were studied.After 30 d of treatment,the rooting plants,the root number of each plant and the root length were added up and the rooting rate was calculated.Result] The rooting rate was 0 when the IAA was used only.The effect of IBA with 1.0 mg/L concn.was the best,its rooting rate was 53.20%,the average root number of each plant was 3.39,and the average root length was 2.84 cm.The rooting rate was 0 when the IAA and the BA was combined with 3 different concn.The effect of the treatment with 0.04 mg/L BA 1.4 mg/L IBA was the best among the combinations of IBA and BA with 3 concn.and the rooting percentage was 73.10%.The effect of IBA with the concn.of 100 mg/L was the best,its rooting percentage was 50.32%,the average number of root was 3.21,and the average root length was 2.26 cm.The rooting effect of the basal part shading was worse than that of darkness culture Conclusion] The rooting rate of the treatment of 0.04 mg/L BA 1.4 mg/L IBA was the highest.