桃蚜对5种新烟碱类杀虫剂及氟啶虫胺腈抗性的快速检测方法

采用玻璃管药膜法，建立了以吡虫啉、啶虫脒、噻虫嗪、噻虫胺、烯啶虫胺5种新烟碱类杀虫剂及氟啶虫胺腈对桃蚜室内敏感品系(SN)的LC90值作为区分剂量，测定桃蚜对6种杀虫剂敏感性变化的方法，并与浸叶法测得的抗性水平进行了相关性分析，验证了利用区分剂量快速测定桃蚜田间种群对6种杀虫剂抗性水平的准确性。结果表明:6种杀虫剂对桃蚜室内敏感品系的LC90值分别为150.01、1 170.81、54.19、951.34、245.98及133.60 ng/cm2。在此区分剂量下，河南省驻马店地区桃蚜种群(ZM)的死亡率在82%~96%之间；河北省玉田地区甘蓝桃蚜种群(GL)的死亡率在35%~82%之间，桃树桃蚜种群(TS)死亡率在3%~30%之间。分析表明，在选定的区分剂量下，桃蚜田间种群的死亡率与其对杀虫剂的抗性水平呈负相关，相关系数在0.818 8~0.999 9之间。同时，通过相关性方程计算得到的江苏省南京地区桃蚜种群(NJ)对6种杀虫剂的理论抗性水平与实际检测所得抗性水平结果接近。因此，以玻璃管药膜法确定的吡虫啉、啶虫脒、噻虫嗪、噻虫胺、烯啶虫胺及氟啶虫胺腈对桃蚜室内敏感品系的LC90值作为区分剂量，通过测定桃蚜田间种群的死亡率，可以快速表征田间种群对6种杀虫剂的敏感性变化，从而对其抗性水平进行初步评估。

The rapid detection method for resistance to five neonicotinoid insecticides and sulfoxaflor in Myzus persicae

Bioassay method of residual film in glass tube was employed to determinate the LC₉₀ values of Myzus persicae susceptible strain (SN) to six insecticides, including imidacloprid, acetamiprid, thiamethoxam, clothianidin, nitenpyram and sulfoxaflor. The LC₉₀ values were then used as the diagnose doses for the detection of the sensitivity variations of M.persicae field populations to the six insecticides. Furthermore, the correlation analysis between the mortalities of M.persicae field populations under the diagnose doses of those six insecticides and the resistance levels obtained by leaf dipping bioassay were conducted. The accuracy of the tested resistance levels of M.persicae field populations to the six insecticides using the diagnose doses were verified. The results showed that the LC₉₀ of SN strain to imidacloprid, acetamiprid, thiamethoxam, clothianidin, nitenpyram and sulfoxaflor, were 150.01, 1 170.81, 54.19, 951.34, 245.98 and 133.60 ng/cm², respectively. Under the treatments of diagnose doses of the six insecticides, the mortality of field population from Zhumadian region of Henan Province (ZM) was between 82% and 96%, the mortality of field population collected from cabbage in Yutian region of Hebei Province (GL) was between 35% and 82%, and the mortality of population collected from peach in the same region (TS) was between 3% and 30%. The correlation analysis showed that the mortalities of M.persicae field populations under the established insecticides diagnose doses were negative correlated with their resistance levels to the six insecticides, with the correlation coefficient between 0.818 8 and 0.999 9. Meanwhile, the theoretical resistance levels of field population from Nanjing region of Jiangsu Province (NJ) to the six insecticides calculated by the correlation equations were close to the actual detected resistance levels. Therefore, through the bioassay method of residual film in glass tube, the LC₉₀ values of the six insecticides to SN strain were established as the diagnose doses. By examining the mortalities of field populations of M.persicae under the diagnose doses, the sensitivity variations of M.persicae field populations to the six insecticides could be rapidly characterized. Moreover, a preliminary assessment of the resistance levels was conducted.