| 芽孢杆菌B96-II对芦笋茎枯病的防治及机制研究 |
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| 引用本文： | 马利平,郝变青,秦曙,乔雄梧.芽孢杆菌B96-II对芦笋茎枯病的防治及机制研究[J].华北农学报,2008,23(2):180-184. |
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| 作者姓名： | 马利平 郝变青 秦曙 乔雄梧 |
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| 作者单位： | 山西省农业科学院,山西省农药重点实验室,山西,太原,030031 |
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| 基金项目： | 山西省科技攻关项目
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山西省重点实验室开放基金 |
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| 摘 要： | 试验表明,芽孢杆菌B96-II对芦笋茎枯病菌(Phomopsis asparagiSacc)菌丝生长的抑制率为89.62%,对孢子产生的抑制率为99.65%;对芦笋茎枯菌的田间防治效果为93.10%。研究表明,B96-II的拮抗蛋白对芦笋茎枯菌有明显的抑制活性;对芦笋的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)均有激活和诱导作用。B96-II处理后30 d,芦笋组织的SOD活力较对照提高254.98%,CAT活力提高81.84%,POD活性提高22.13%,PPO活力提高146.67%,PAL活力提高171.60%。
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| 关 键 词： | 芦笋茎枯病 芽胞杆菌 拮抗蛋白 诱导抗性 保护酶 |
| 文章编号： | 1000-7091(2008)02-0180-05 |
| 修稿时间： | 2007年12月7日 |
Causal Study on Inhibition Effects of Antagonistic Bacillus Strain B96-II to Asparagus Stem Blight |
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| MA Li-ping,HAO Bian-qing,QIN Shu,QIAO Xiong-wu.Causal Study on Inhibition Effects of Antagonistic Bacillus Strain B96-II to Asparagus Stem Blight[J].Acta Agriculturae Boreali-Sinica,2008,23(2):180-184. |
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| Authors: | MA Li-ping HAO Bian-qing QIN Shu QIAO Xiong-wu |
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| Abstract: | Antagonistic Bacillus strain B96-II could effectively inhibite mycelia growth and spore germination of Phomopsis asparagi Sacc,pathogen of asparagus stem blight.The inhibition rates were 89.62% and 99.65% respectively.Field trials showed a control efficacy of 93.10%.Contents of 5 resistance related enzymes in asparagus plants were stimulated distinctly 30 d after treatment with B96-II.Compared with untreated control sample,superoxide dismutase activity had an increase of 254.98%,such as 81.84% to catalase,22.13% to peroxidase,146.67% to polyphenoloxidase,and 171.60% to phenylalanin ammonialyase. |
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| Keywords: | Asparagus stem blight Bacillus sp Antagonistic protein Induced resistance Protective enzyme |
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