焦锑酸钾沉淀法定位Ca~(2+)在梨生理研究中的应用 |
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引用本文: | 彭抒昂. 焦锑酸钾沉淀法定位Ca~(2+)在梨生理研究中的应用[J]. 果树学报, 2001, 0(3) |
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作者姓名: | 彭抒昂 |
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作者单位: | 华中农业大学园艺系!湖北武汉 430070 |
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基金项目: | 国家自然科学基金资助项目!39870517 |
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摘 要: | 介绍了应用焦锑酸钾沉淀法定位Ca2+的方法,以及在梨生理研究中的具体步骤和应用效果。结果表明:在固定液中加焦锑酸钾处理进行制样,梨树不同器官或组织细胞中的自由态Ca2+,都能生成焦锑酸钙在原部位沉淀,经染色后在电子显微镜下呈现出黑色颗粒,而固定液中不加焦锑酸钾处理的则没有这种颗粒。再进一步用Ca2+的专一性鳌合物EGTA进行处理,检查这些黑色颗粒是否消失,以证实黑色颗粒就是Ca2+,从而可对Ca2+进行定位标记。在不同的组织细胞及同一组织细胞在不同的生理时期,其被标记的Ca2+密度和场所是不同的,可通过其动态变化来探讨它的作用机理。还对用此方法定位Ca2+的注意事项进行了讨论。
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关 键 词: | Ca~(2+)定位 焦锑酸钾沉淀法 梨 生理 |
Localization of Ca(2+) by Antimonite Precipitation Technique and Its Application to Pear Physiological Research |
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Abstract: | The method of localising Ca2+ by antimonite precipitation technique the detailed procedure and effects of its application to fruit physiological research were presented in this paper. The results showed that with treatment of the fixative solution added with antimonite the free Ca2+ in pear different organs and tissues could be changed into antimonite calcium and precipitated in situ which after being stained appeared as black particles under electronic microscope whereas such particles could not be observed in the treatment without antimonite. Furthermore the samples were treated with EGTA a specific reagent in order to see whether the black particles would disappear and to confirm that the black particles were Ca2+ so that Ca2+ could be localised and marked. In different tissues and during different physiological periods of the same tissue the density and site of the marked Ca2+ were different which could be used to study the mechanism of Ca2+ by means of its fluctuation. The problems to which we should pay attention when the localization of Ca2+ was conducted by this method were also discussed. |
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Keywords: | Localization of Ca2+ Antimonite precipitation technique Pear;Physiology |
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