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Oligoribonucleotides: improvement in chromatographic separation |
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| Authors: | Ishikura H Neelon F A Cantoni G L |
| Abstract: | The separation of mixed oligoribonucleotides obtained from enzymatic digestion of soluble RNA into fractions containing mixed oligonucleotides of identical chain length is improved by absence of terminal phosphate. Each fraction may be separated into individual components by ionexchange (Dowex) chromatography. Combination of these two chromatographic procedures yields maximum information on primary structure of the oligonucleotides. |
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