| 丛枝菌根真菌光自养培养体系的建立 |
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| 引用本文： | 陈浩,肖翔,王军,吴李君. 丛枝菌根真菌光自养培养体系的建立[J]. 安徽农业科学, 2010, 0(25): 13605-13606. DOI: 10.3969/j.issn.0517-6611.2010.25.020 |
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| 作者姓名： | 陈浩 肖翔 王军 吴李君 |
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| 作者单位： | 中国科学院离子束生物工程学重点实验室,安徽合肥,230031;中国科学技术大学化学与材料科学学院,安徽合肥,230026 |
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| 基金项目： | 中国科学院合肥物质科学研究院院长基金 |
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| 摘 要： | [目的]建立以蒺藜苜蓿（Medicago truncatula）为寄主的丛枝菌根真菌光自养培养体系。[方法]以丛枝菌根真菌（Glomus etunica-tum）为试验材料,以豆科模式植物——蒺藜苜蓿为寄主,建立植物根系在严格的培养条件下与AMF的共生关系,而植物的地上部分可以进行正常的光合作用,并对孢子产量、根的侵染情况以及后代孢子的活力进行了评估。[结果]孢子能正常侵染植物并产生大量新生孢子,新生孢子在相同条件下可以继代培养。[结论]本研究为在光自养条件下多方位研究菌根真菌提供了实验平台。
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| 关 键 词： | 光自养培养 蒺藜苜蓿 丛枝菌根真菌 |
Establishment of a Photoautotrophic Culture System for the in vitro Mycorrhization of Medicago truncatula Plantlets |
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| CHEN Hao et al. Establishment of a Photoautotrophic Culture System for the in vitro Mycorrhization of Medicago truncatula Plantlets[J]. Journal of Anhui Agricultural Sciences, 2010, 0(25): 13605-13606. DOI: 10.3969/j.issn.0517-6611.2010.25.020 |
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| Authors: | CHEN Hao et al |
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| Affiliation: | CHEN Hao et al (Key Laboratory of Ion Beam Bioengineering,Institute of Plasma Physics,Chinese Academy of Science,Hefei,Anhui 230031) |
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| Abstract: | [Objective]The aim is to establish an photoautotrophic culture system for the in vitro mycorrhization of Medicago truncatula plantlets.[Method]With the arbuscular mycorrhizal fungi Glomus etunicatum as the experiment material and Leguminous model plant-Medicago truncatula as the host,the Photoautotrophic culture system,under which a symbiosis between plant roots and AMF is established under in vitro conditions while shoots developed under open air conditions,is established.Moreover,the spore yield,root infe... |
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| Keywords: | Arbuscular mycorrhizal fungi Autotrophic culture Medicago truncatula |
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