杨尺蠖核多角体病毒的研究——Ⅴ.AciNPV杀虫剂的研制及产品检测

本文报道 AciNPV杀虫剂的生产工艺和安全性检测结果。AciNPV杀虫剂是以多角体制备物加光保护剂和其它助剂,在电动混合筛内混合制备而成。生测证明,丙酮—乳糖共沉样品与病毒杀虫剂对幼虫的致病力很高。其LC_(50)均为2.84×10~3PIB/ml;95％置信限分别为1.70×10~3—4.76×10~3 PIB/ml 或1.43×10~3—5.60×10~3 PIB/ml。 几种粉剂可直接用水和矿物油(如零号柴油)稀释,使用较方便。野外试验表明,防治效果在85％以上。 动物安全性试验表明,该制剂无致病性和致突变作用,对人畜安全。细菌学检测证明,未发现任何与人有关的致病菌,如大肠杆菌、沙门氏菌、志贺菌、弧菌和破伤风杆菌等。

STUDIES ON THE NUCLEAR POLYHEDROSIS VIRUS OF THE POPLAR LOOPER (APOCHEIMA CINERARIUS) V.PREPARATION AND PRODUCT DETECTION OF ACINPV FORMULATION

The present paper reports the processing techiniques of the formulation of nuclear polyhedrosis virus of Apocheima cinerarius, and its detection of safety.New formulation of the AciNPV were made by incorporating the preparation of polyhedrals inclusion bodies (PIB) with sunlight protectant and other abjuvants in a blend screen of electroshak.Bioassays tests indicated that samples of the aceton-precipitation and new formulations of virus were very effective for early third-instar larvae.The LC50 and 95% fiducial limits were 2.84×10³PIB/ml, 2.84×10³PIB/ml, and 1.70×10³-4.76×10³ PIB/ml and 1.43×10³-5.66×10³PIB/ml respectively.Several powder formulations were directly diluted with water and mineral oil (NO.0 diesel oil) when used in the field.The tests conducted on small plots and large blocks showed that several formulations of AciNPV were effective for the poplar looper 2-3 instar larvae in forest field.The mortality of poplar looper larvae was over 85% in 12-15 days.Safety tests showed that formulation of AciNPV was not hamper to domestic animals and did not couse mutation.It was safety for man.Bacteriological detection proved that pathogenic bacteria, such as Escherichia coli, Salmonell, Vibric, and Clostridium were cultured without isolation.