| Abstract: | The second internal transcribed spacer (ITS-2) of Leucocytozoon eaulleryi was amplified byPCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. Theinserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2-+-) wascomposed of 270 nucleotides, and the ITS-2 was 113 bp in length. The ITS-2 of L. eaulleryi was analysed byNCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeriatenella, Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that theITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%). |
