毛竹cab-PhE11基因的克隆与序列分析

采用RT-PCR技术和RACE-PCR技术,从毛竹(Phyllostachys edulis)中分离了捕光叶绿素a/b结合蛋白基因cDNA编码区全长,命名为cab-PhE11(GenBank登记号:EU327783)。该基因全长1154 bp,编码区cDNA全长753 bp,编码250个氨基酸。生物信息学分析表明,在cab-PhE11编码的蛋白第62~239位包括典型的捕光叶绿素a/b结合蛋白功能域,还包含1个N-糖基化位点、1个酪氨酸激酶Ⅱ磷酸化位点、5个N-肉豆蔻酸化位点以及1个丙氨酸富集区。序列相似性分析结果表明,cab-PhE11编码的氨基酸序列与玉米、绿豆、拟南芥、烟草等的cab基因有较高的相似性,都在80%以上,该基因属于lhcb6类基因。

Cloning and sequence analysis of a full-length cDNA encoding light-harvesting chlorophyll a/b- binding protein of PSII in Moso

A full-length cDNA of light-harvesting chlorophyll a/b(cab) gene was cloned from the first strand of Moso(Phyllostachys edulis) cDNA through RT-PCR and RACE-PCR methods,named as cab-PhE11(GenBank accession number:EU327783).The length of cab-PhE11 is 1154 bp,which contains an open reading frame(753 bp) encoding 250 amino acids.The bioinformatics analysis indicated that the protein encoded by cab-PhE11 had one chlorophll a/b binding domain(62th～239th position),one N-glycosylation site,one Casein kinase II phosphorylation site,five N-myristoylation site and one alanine-rich region profile.The amino acid sequence of cab-PhE11 showed high similarity with the cab genes of Zea mays,Vigna radiata,Arabidopsis thaliana and Nicotiana tabacum,more than 80% respectively,which indicated that cab-PhE11 gene belongs to lhcb6 gene family.