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Radical Scavenging Activity and Stability of Betalains from <Emphasis Type="Italic">Beta vulgaris</Emphasis> Hairy Root Culture in Simulated Conditions of Human Gastrointestinal Tract
Authors:Email author" target="_blank">Atanas?PavlovEmail author  Petia?Kovatcheva  Dimka?Tuneva  Mladenka?Ilieva  Thomas?Bley
Institution:(1) Institut für Lebensmittel und Bioverfahrnstechnik, Technische Universität Dresden, Mommsenstraβe 13, 01069 Dresden, Germany;(2) Groupe of Biological Active Substances from Plant in vitro Systems, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria
Abstract:Stability and radical scavenging activity of betalains, biosynthesized by Beta vulgaris hairy root culture were investigated at different pH values, bile salts concentration, as well as at the in vitro conditions of gastrointestinal tract. It was established that pH below 3 and concentrations of the bile salts up to 4% had no great influence on betalains stability. At the in vitro conditions of gastrointestinal tract betalains are relatively stable, as their radical scavenging activity decrease from 75% inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) to about 38%.
Keywords:Betalains  Beta vulgaris hairy root culture  In vitro conditions of gastrointestinal tract  Radical scavenging activity
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