罗非鱼"突眼"病细菌性病原的分离与鉴定

从患"突眼"症状的罗非鱼体内分离到多株细菌,经人工感染证实其中TL60829NA菌株为病原菌,经生理、生化和API细菌鉴定系统以及分子生物学试验,鉴定其为痢疾志贺氏菌(Shigella dysenteriae)。其主要生理生化特征为革兰氏阴性短杆菌,不具动力,氧化酶、过氧化氢酶、V-P反应、吲哚试验、苯丙氨酸、甘露醇、KCN均为阴性;对氨苄西林、羧苄西林、红霉素、多西环素、氟哌酸、卡那霉素敏感,对呋喃唑酮、复方新诺明、磺胺和庆大霉素不敏感。

Isolation and Identification of Pathogenic Bacteria from Exophthalmic Disease of Tilapia

The bacterium strains were isolated from the exophthalmic disease of tilapia,one of the isolates were proved to be the pathogens of the disease.By challenge test,it was identified to be the Shigella dysenteriae with the combination of molecular biology experiments.The bacteria are gram-negative and not motive short rods.The physiological and biochemical characteristics were as the follows:oxidase,catalase,V-P reaction,indole reaction,phenylalaninase,mannitol and KCN were all negative.The bacterium is highly sensitive to ampicillin,carbenicillin,erythromycin,doxycycline,norfloacin,kanamycin,not furazolidone,sulfamethoxazole/time thoprin,gentamycin and sulfamethoxazole.