| 湖北省柑桔裂皮类病毒的检测及序列分析 |
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| 引用本文： | 何云蔚,洪霓,徐文兴,王国平. 湖北省柑桔裂皮类病毒的检测及序列分析[J]. 华中农业大学学报, 2007, 26(3): 289-292 |
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| 作者姓名： | 何云蔚 洪霓 徐文兴 王国平 |
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| 作者单位： | 华中农业大学植物科技学院,武汉,430070;华南农业大学资源环境学院,广州,510642;华中农业大学植物科技学院,武汉,430070 |
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| 基金项目： | 科技部863项目(2001AA241142)资助 |
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| 摘 要： | 以表现柑桔裂皮病典型症状的罗伯逊脐橙为材料,采用双向电泳、RT-PCR和RNA斑点杂交及组织印迹杂交的方法,对柑桔裂皮类病毒进行了检测,结果表明RT-PCR和2种核酸杂交技术均可用于该类病毒的有效检测。对该类病毒全长RNA的RT-PCR产物进行了克隆和序列分析,该分离株(命名为CEVd-HB分离株)的全长RNA由371个核苷酸组成,与已报道的其它不同来源分离株相似性为90%~99%。
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| 关 键 词： | 柑桔裂皮类病毒 检测 克隆 序列分析 |
| 文章编号： | 1000-2421（2007）03-0289-04 |
| 修稿时间： | 2006-11-142007-03-19 |
Detection and Analysis of Genomic RNA Sequence of Citrus exocortis viroid from Hubei Province |
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| HE Yun-wei,HONG Ni,XU Wen-xing,WANG Guo-ping. Detection and Analysis of Genomic RNA Sequence of Citrus exocortis viroid from Hubei Province[J]. Journal of Huazhong Agricultural University, 2007, 26(3): 289-292 |
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| Authors: | HE Yun-wei HONG Ni XU Wen-xing WANG Guo-ping |
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| Affiliation: | 1. College of Plant Science and Technology, Huazhong Agricultual University ,Wuhan 430070, China; 2. College of Resources and Envronment , South China Agrcultural University, Guangzhou 510642, China |
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| Abstract: | Citrus exocortis viroid(CEVd) in Citrus sinensis Osbeck cv.Robertson,which showed typical symptoms of CEVd infection,were detected by bi-directional PAGE electrophoresis,RT-PCR,RNA dot-blotting and tissue-printing hybridization.Results showed that RT-PCR and two hybridization methods were effective for the detection of the viroid.The RT-PCR products from the full-length genomic RNA of the viroid(named as CEVd-HB) were cloned.Results of sequence analysis indicated that the full-length genomic RNA of the viroid consist of 371 nucleotide acids and had similarities ranging from 90% to 99% with reported isolates of the viroid. |
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| Keywords: | Citrus exocortis viroid detection clone sequence analysis |
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