| 高山红景天GH3基因克隆及序列分析 |
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| 引用本文： | 张继星,庞晓斌,于寒松,王慧然,李彦舫. 高山红景天GH3基因克隆及序列分析[J]. 安徽农学通报, 2007, 13(23): 30-31,44. DOI: 10.3969/j.issn.1007-7731.2007.23.013 |
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| 作者姓名： | 张继星 庞晓斌 于寒松 王慧然 李彦舫 |
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| 作者单位： | 内蒙古民族大学生命科学学院,内蒙古通辽,028043;河南大学药学院,河南开封,475001;吉林大学植物科学学院,吉林长春,130062;包头市园林处银河广场管理所,内蒙古包头,014030 |
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| 基金项目： | 国家自然科学基金,编号:30471229 |
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| 摘 要： | 参照已知植物GH3的氨基酸序列,通过比较找出相对保守的氨基酸序列设计简并引物,通过RT-PCR和RACE法得到高山红景天GH3的3'端,其长度838bp,编码200个氨基酸的602 bp位于GH3的开放阅读框内和一段长36bp的3'非编码区(3'UTR).序列比较结果表明,GH3推导的氨基酸序列与已公布的几种植物GH3氨基酸序列有较高的序列同源性.
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| 关 键 词： | 高山红景天 GH3基因 克隆 序列分析 |
| 文章编号： | 1007-7731（2007）23-30-02 |
| 收稿时间： | 2007-12-05 |
| 修稿时间： | 2007-12-05 |
Cloning and Sequence Analysis of GH3 from Rhodiola Sachalinensis A.Bor |
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| Zhang Ji xing,Pang Xiaobin,Yu Hansong,Wang Huirang,Li Yangfang. Cloning and Sequence Analysis of GH3 from Rhodiola Sachalinensis A.Bor[J]. Auhui Agricultural Science Bulletin, 2007, 13(23): 30-31,44. DOI: 10.3969/j.issn.1007-7731.2007.23.013 |
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| Authors: | Zhang Ji xing Pang Xiaobin Yu Hansong Wang Huirang Li Yangfang |
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| Abstract: | The primer was designed referring to the conservative amino acid sequences of known plant GH3.The partial sequence of GH3 were amplified from Rhodiola sachalinensis A.Bor by RT-PCR and RACE respectively.The results as follows:the length of cloned GH3 cDNA is 838bp,which is comprised in open reading frame(ORF) of 602 bp encoding 200 amino acids and untranslated region(3'UTR) of 36 bp.The results of sequence comparison indicated that the amino acids sequence deduced from the nucleotide sequence of GH3 form Rhodiola sachalinensis A.Bor shares high sequence homology with the selected GH3 amino acids of plants. |
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| Keywords: | Rhodiola sachalinensis A.Bor GH3 Cloning Sequence Analysis |
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