| 根癌农杆菌介导豇豆胰蛋白酶抑制剂基因转入苹果主栽品种 |
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| 引用本文： | 师校欣,王斌,杜国强,翁曼丽,高仪,马宝□,邵建柱,贾建航.根癌农杆菌介导豇豆胰蛋白酶抑制剂基因转入苹果主栽品种[J].园艺学报,2000,27(4):282-284. |
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| 作者姓名： | 师校欣 王斌 杜国强 翁曼丽 高仪 马宝□ 邵建柱 贾建航 |
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| 作者单位： | 1. 河北农业大学园艺系,保定071001
2. 中国科学院遗传研究所,北京100101 |
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| 基金项目： | 河北省自然科学基金资助项目! (3971 4 6 )，河北省教委博士基金资助项目 |
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| 摘 要： | 研究建立起一套简单、高效的苹果遗传转化系统。利用根癌农杆菌介导的叶圆片转化法将高效启动的豇豆胰蛋白抑制剂基因（ＣｐＴｌ）转入富士、齐纳金、王林、嘎拉等苹果主栽品种中，经卡那霉素抗性、ＰＣＲ、点杂交、Ｓｏｕｔｈｅｍ杂交检测证明了４个品种均获得转化再生植株，现已移栽成活。
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| 关 键 词： | 苹果 抗中性 遗传转化 豇豆胰蛋白酶抑制剂基因 |
| 修稿时间： | 2000-01-03 |
Studies on Agrobacterium-mediated CpTI Gene Tranfer in Commercial Apple Cultivars |
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| Shi Xiaoxin ,Wang Bin ,Du Guoqiang ,Wong Manli ,and Gao Yi.Studies on Agrobacterium-mediated CpTI Gene Tranfer in Commercial Apple Cultivars[J].Acta Horticulturae Sinica,2000,27(4):282-284. |
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| Authors: | Shi Xiaoxin Wang Bin Du Guoqiang Wong Manli and Gao Yi |
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| Institution: | Shi Xiaoxin 1,Wang Bin 2,Du Guoqiang 1,Wong Manli 2,and Gao Yi 1 |
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| Abstract: | A simple and efficient genetic transfer system was developed in apple.CpTI gene was introduced into commercial apple cultivars(i.e.Fuji,Jonagold,Orin,Royal Gala)in vitro using Agrobacterium tumefaciens mediated. The plantlets,which regenerated from leaf disc and grown normally on Km medium,were confirmed to bring CpTI gene by PCR dot blot,and Sonthern blot analysis.Some of them were transferred into greenhouse successfully. |
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| Keywords: | Apple Agrobacterium tumefaciens CpTI gene Promoter Transformation |
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