| Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia ‘Idaho’ |
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| 作者姓名： | Xie Jim-wen Li Min Chen Shou-yi Sun De-you Wang Hua-fang College of Biological Sciences and Biotechnology Beijing Forestry University Beijing P. R. China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing P. R. China Grape Juice Manufactory of Changbai Mountains Ji'an P. R. China |
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| 作者单位： | Xie Jim-wen Li Min Chen Shou-yi Sun De-you Wang Hua-fang College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China Institute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，P. R. China Grape Juice Manufactory of Changbai Mountains，Ji'an 134200，P. R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家重点基础研究发展计划(973计划) |
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| 摘 要： | Robinia pseudoacacia 'Idaho' (Robinia×ambigua 'Idahoensis', R. pseudoacacia×R. viscosa) modified by a mtl-D gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by previous investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg·L-1 IB A and 0.1 mg·L-1 NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.
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| 关 键 词： | Robinia pseudoacacia ‘Idaho’ rooting acclimatization micropropagation in vitro |
| 收稿时间： | 2006-6-4 |
Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia 'Idaho' |
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| Xie Jim-wen Li Min Chen Shou-yi Sun De-you Wang Hua-fang College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing ,P. R. China Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing ,P. R. China Grape Juice Manufactory of Changbai Mountains,Ji''''an ,P. R. China.Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia ''''Idaho''''[J].Forestry Studies in China,2006,8(4):69-74. |
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| Authors: | Xie Jun-wen Li Min Chen Shou-yi Sun De-you Wang Hua-fang |
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| Institution: | 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P. R. China 3Grape Juice Manufactory of Changbai Mountains, Ji’an 134200, P. R. China |
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| Abstract: | Robinia pseudoacacia 'Idaho' (Robinia × ambigua 'Idahoensis', R. pseudoacacia × R. viscosa) modified by a mtl-D gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by previous investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory.For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field. |
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| Keywords: | Robinia pseudoacacia 'Idaho' rooting acclimatization micropropagation in vitro |
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