无机盐、维生素及生长调节剂对斑玉蕈菌丝生长的影响

【目的】寻找对斑玉蕈菌丝生长有促进作用的无机盐、维生素及植物生长调节剂，为斑玉蕈液体菌种的进一步开发与利用提供参考。【方法】以斑玉蕈菌株HM-2为材料，向培养基中加入不同种类及质量浓度的无机盐、维生素和植物生长调节剂，于（23±1） ℃、空气相对湿度70%Hypsizigus marmoreus (Peck) Bigelow;inorganic salts;vitamins;plant hormones 75% 的条件下培养12 d后测定菌落直径，研究上述物质对斑玉蕈菌丝生长的影响。【结果】与不添加任何无机盐的培养基相比，0.5～2.0 g/L的 MgSO₄、NaCl和KCl对斑玉蕈菌丝生长均有一定的促进作用，且均以1.5 g/L时促进作用最大，菌落直径分别达到81.04，78.93和80.29 mm；而0.5～2.0 g/L 的CaCl₂、KH₂PO₄和0.01Hypsizigus marmoreus (Peck) Bigelow;inorganic salts;vitamins;plant hormones 0.04 g/L的ZnSO₄对斑玉蕈菌丝的生长具有不同程度的抑制作用。与不添加任何维生素的培养基相比，2.0Hypsizigus marmoreus(Peck) Bigelow;inorganic salts;vitamins;plant hormones 10.0 mg/L的烟酸、叶酸、V_B1和V_B2对斑玉蕈菌丝生长均有一定的促进作用，以2.0 mg/L叶酸的促进作用最大，菌落直径达到67.76 mm；而2.0～10.0 mg/L的V_B6对斑玉蕈菌丝生长无明显的促进和抑制作用。与不添加任何植物生长调节剂的培养基相比，2.0～10.0 mg/L的IBA、GA₃和NAA对斑玉蕈菌丝生长均有极显著的促进作用，以2.0 mg/L IBA的促进作用最大，菌落直径达到69.33 mm；而2.0～10.0 mg/L的KT与6-BA对斑玉蕈菌丝生长的促进作用相对较弱。【结论】在培养基中添加适量的MgSO₄、NaCl、KCl、烟酸、叶酸、V_B1、V_B2、IBA、GA₃和NAA，可促进斑玉蕈菌丝的生长。

Effect of several different inorganic salts,vitamins and plant hormones on the mycelial growth of Hypsizigus marmoreus(Peck) Bigelow

【Objective】The research was made to look for several different kinds of inorganic salts,vitamins and plant hormones which have definite promotion on the mycelial growth of Hypsizigus marmoreus(Peck) Bigelow,in order to provide reference information for development and application of H.marmoreus(Peck) Bigelow liquid cultivation.【Method】H.marmoreus(Peck) Bigelow HM-2 was taken as test material,which was cultivated in culture mediums with different kinds of inorganic salts,vitamins and plant hormones under the temperature of(23±1) ℃,RH 70%-75%,and then the colony sizes of the different culture mediums were measured after twelve days to research the effect of different kinds of inorganic salts,vitamins and plant hormones with different concentrations on the growth of H.marmoreus(Peck) Bigelow mycelium.【Result】Compared with no inorganic salts applicated culture mediums,0.5-2.0 g/L magnesium sulfate,sodium chloride and potassium chloride have definite promotion on the mycelium growth of H.marmoreus(Peck) Bigelow,the biggest colony size of H.marmoreus(Peck) Bigelow is 81.04 mm,78.93 mm and 80.29 mm respectively in the concentration of 1.5 g/L,but 0.5-2.0 g/L calcium chloride,potassium dihydrogen phosphate and 0.01-0.04 g/L zinc sulfate have different degrees of inhibition on the mycelium growth of H.marmoreus(Peck) Bigelow.Compared with no vitamins applicated culture mediums,2.0-10.0 mg/L Nicotinic acid,folic acid,VB1 and VB2 promote the mycelium growth of H.marmoreus(Peck) Bigelow,the biggest colony size of H.marmoreus(Peck) Bigelow is 67.76 mm in the folic acid concentration of 2.0 mg/L,but VB6 has neither promotion nor inhibition on the mycelium growth of H.marmoreus(Peck) Bigelow.Compared with no plant hromones applicated culture mediums,2.0-10.0 mg/L IBA,GA3 and NAA of plant hormones have prominent promotion on the mycelium growth of H.marmoreus(Peck) Bigelow,the biggest colony size of H.marmoreus(Peck) Bigelow is 69.33 mm in the IBA concentration of 2.0 mg/L,but 2.0-10.0 mg/L KT and 6-BA have less effect on the mycelium growth of H.marmoreus(Peck) Bigelow in comparion to the other plant hormones.【Conclusion】Adding moderate concentrations of magnesium sulfate,sodium chloride,potassium chloride,nicotinic acid,folic acid,VB1,VB2,IBA,GA3 and NAA could enhance the mycelium growth of H.marmoreus(Peck) Bigelow.