| 水分胁迫诱导八棱海棠和平邑甜茶细胞程序性死亡的研究 |
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| 引用本文： | 曹慧,李春霞,王孝威,邹岩梅,束怀瑞.水分胁迫诱导八棱海棠和平邑甜茶细胞程序性死亡的研究[J].园艺学报,2009,36(4):469-474. |
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| 作者姓名： | 曹慧 李春霞 王孝威 邹岩梅 束怀瑞 |
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| 作者单位： | (山东农业大学园艺科学与工程学院, 山东泰安271018 ; 潍坊学院, 山东潍坊261061; 山西农业大学, 山西太谷 030801; 延安职业技术学院, 陕西延安716000) |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金，山西省自然科学基金 |
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| 摘 要： | 以苹果属抗旱能力较强的八棱海棠(Malus robusta Rehd. ) 和较弱的平邑甜茶Malus hupehensis (Pamp.) Rehd. ] 水培幼苗为试验材料, 利用细胞形态学、DNA ladder等技术, 用20% PEG 6000模拟水分胁迫进行诱导细胞程序性死亡研究。经DAP I染色后荧光显微镜观察发现两个苹果属材料均呈现细胞染色质凝集、细胞核缩小、变形、解体等细胞程序性死亡的形态学特征; 同时, DNA琼脂糖凝胶电泳可观察到明显的“梯状”DNA条带(DNA ladder) 。由此表明, 水分胁迫诱导苹果属植物具有明显的细胞程序性死亡的典型形态及生化特征, 是一种主动的细胞死亡过程, 八棱海棠细胞程序性死亡持续的时间长于平邑甜茶。
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| 关 键 词： | 苹果属 水分胁迫 程序性死亡 DAPI DNA ladder |
| 收稿时间： | 2008-10-14 |
| 修稿时间： | 2009-4-1 |
Research of Programmed Cell Death Under Water Stress in Malus robusta Rehd. and Malus hupehensis (Pamp.) Rehd. |
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| CAO Hui,LI Chun-xia,WANG Xiao-wei,ZOU Yan-mei,SHU Huai-rui.Research of Programmed Cell Death Under Water Stress in Malus robusta Rehd. and Malus hupehensis (Pamp.) Rehd.[J].Acta Horticulturae Sinica,2009,36(4):469-474. |
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| Authors: | CAO Hui LI Chun-xia WANG Xiao-wei ZOU Yan-mei SHU Huai-rui |
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| Institution: | ( Institute of Horticultural Science and Engineering, Shandong Agricultural University, Taipan, Shandong 271018, China;Weifang University, W eifang, Shandong 261061, China; Shanx i Agricultural University, Taigu, Shanx i 030801, China;Yanan Vocational and Technical College, Yanan, Shaanx i 716000, China) |
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| Abstract: | The seedling of Malus robusta Rehd. and Malus hupehensis ( Pamp. ) Rehd. were treated with 20% PEG 6000. The study was to observe p rogrammed cell death under water stress through morphocytology and DNA ladder. The nuclearmorphology was observed after DAP I staining. And the results showed that the characteristic morphological changes of chromatin /chromosome and nuclear such as chromatin condensed, karyon shinked, distorted and degraduated were found with stress time. Meantime, DNA ladder was found in cells after agarose gel electrophoresis. Cell death induced bywater stress showed typ icalmorphological and biochemical p roperties of p rogrammed cell death, and thiswas an active cell death. Additionally, PCD in Malus robusta Rehd. was longer than in Malus hupehensis ( Pamp. ) Rehd. seedlings. |
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| Keywords: | DAPI DNA ladder |
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