敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达研究 |
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引用本文: | 赵金山,李和刚,刘开东,柳楠,李金泉. 敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达研究[J]. 安徽农业科学, 2012, 0(30): 14731-14733,14740 |
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作者姓名: | 赵金山 李和刚 刘开东 柳楠 李金泉 |
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作者单位: | 内蒙古农业大学,内蒙古呼和浩特010018;山东省青岛市畜牧兽医研究所,山东青岛266100;山东省青岛市畜牧兽医研究所,山东青岛,266100;青岛农业大学,山东青岛,266019;内蒙古农业大学,内蒙古呼和浩特,010018 |
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基金项目: | 国家绒毛用羊产业技术体系资助项目(CARS-40) |
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摘 要: | [目的]从免疫基因中挖掘调控羊毛性状的候选主效基因。[方法]利用芯片技术,检测敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达。[结果]差异倍数在2.0以上的免疫基因共46个,并对其中6个基因进行了实时定量PCR验证,其结果表明芯片数据和实时定量PCR验证结果相符率达66.67%,说明芯片数据是可靠的。[结论]免疫赦免机制可能参与羊毛的生长调控。
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关 键 词: | 敖汉细毛羊 毛囊 免疫基因 差异表达 |
Study on the Differential Expression of Immune Genes between Body Side Skin and Groin Skin of Aohan Fine Wool Sheep |
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Affiliation: | ZHAO Jin-shan et al(Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018) |
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Abstract: | [Objective] The research aimed to find out candidate major genes for regulating the wool traits from immune genes.[Method] Using microarray technology,the differential expression of immune genes between body side skin and groin skin of Aohan fine wool sheep was detected.[Result] 46 immune genes(difference multiple)>2.0) were identified and classified.And 6 of them were validated by real-time quantitative PCR.The results showed that the consistency between microarray data and real-time quantitative PCR results was 66.67%,which indicated that the microarray data was reliable.[Conclusion] The mechanism might be involved in the growth regulation of wool. |
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Keywords: | Aohan fine wool sheep Hair follicle Immune gene Differential expression |
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