| 高效液相色谱法测定小麦中阿魏酸的含量 |
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| 引用本文： | 魏岚,颜振敏.高效液相色谱法测定小麦中阿魏酸的含量[J].安徽农业科学,2012,40(28):13693+13728. |
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| 作者姓名： | 魏岚 颜振敏 |
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| 作者单位： | 四川农业大学生命科学与理学院,四川雅安,625014;河南科技学院食品学院,河南新乡,453003 |
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| 摘 要： | 目的]建立HPLC测定小麦中阿魏酸含量的检测方法。方法]色谱柱为Hyperclone BDS C18柱(150 mm×4.60 mm×5μm,phe-nomenex);流动相为甲醇酸性水溶液(V甲醇∶V水∶V冰乙酸=25∶75∶0.75);流速为1.0 ml/min;进样量为20μl;检测波长为320 nm;柱温为30℃。结果]阿魏酸进样浓度在80.8～242.4μg/ml范围内;在选定色谱条件下阿魏酸线性关系良好。结论]该方法操作简单,样品出峰时间较其他方法快,可应用于小麦中阿魏酸含量的测定。
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| 关 键 词： | 苋菜红 果绿 分光光度法 支持向量回归 同时测定 |
A HPLC-DAD Method for Determining Ferulic Aid in Wheat |
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| Institution: | WEI Lan et al(College of Life and Basic Sciences,Sichuan Agricultural University,Ya′an,Sichuan 625014) |
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| Abstract: | Objective] To establish a HPLC method for the determination of ferulic acid in wheat.Method] The HPLC conditions were: Hyperclone BDS C18(150 mm×4.60 mm×5 μm,phenomenex),mobile phase of carbinol acidic water(Vcarbinol∶ Vwater∶ Vglacial acetic acid=25∶ 75∶ 0.75),flow rate of 1.0 ml/min,sampling amount of 20 μl,detection wavelength of 320 nm and column temperature of 30 ℃.Result] In the range of 80.8-242.4 μg/ml,the relationship between concentration of quercetin and peak area was good(r=0.999 7).Conclusion] By comparison with other methods,the method proposed in this paper has the characters of quick peak flowing out.This method can be used to determine ferulic acid in wheat. |
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| Keywords: | Amaranth Fruit green Spectrophotometry Support vector regression Simultaneous determination |
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